Characterization of internalin genes in Listeria monocytogenes from food and humans, and their association with the invasion of Caco-2 cells
| Autor: | Haijian Pan, Xuebin Xu, Guojie Cao, Xiaowei Yang, Jianmin Zhang, Jianghong Meng, Xianming Shi, Dao-Feng Zhang, Dai Kuang, Xudong Su |
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| Rok vydání: | 2019 |
| Předmět: |
inlA
0301 basic medicine Serotype L. monocytogenes 030106 microbiology Virulence Single-nucleotide polymorphism Biology medicine.disease_cause Microbiology Internalins 03 medical and health sciences Invasion Listeria monocytogenes Virology PMSC mutation medicine Internalin lcsh:RC799-869 Gene Mutation Gastroenterology bacterial infections and mycoses 030104 developmental biology Infectious Diseases Parasitology lcsh:Diseases of the digestive system. Gastroenterology |
| Zdroj: | Gut Pathogens, Vol 11, Iss 1, Pp 1-10 (2019) |
| ISSN: | 1757-4749 |
| Popis: | Background Internalins are surface proteins that are utilized by Listeria monocytogenes to facilitate its invasion into human intestinal epithelial cells. The expression of a full-length InlA is one of essential virulence factors for L. monocytogenes to cross the intestinal barrier in order to invade epithelial cells. Results In this study, the gene sequences of inlA in 120 L. monocytogenes isolates from food (n = 107) and humans (n = 13) were analyzed. Premature stop codon (PMSC) mutations in inlA were identified in 51 isolates (50 from food and 1 from human). Six mutation types of PMSCs were identified. Among the 51 isolates with PMSCs in inlA, there were 44 serogroup 1/2c, 3c isolates from food, of which seven belonged to serogroups 1/2a, 3a. A total of 153,382 SNPs in 2247 core genes from 42 genomes were identified and used to construct a phylogenetic tree. Serotype 1/2c isolates with inlA PMSC mutations were grouped together. Cell culture studies on 21 isolates showed that the invasion to Caco-2 cells was significantly reduced among isolates with inlA PMSC mutations compared to those without PMSC mutations (P |
| Databáze: | OpenAIRE |
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