Determination of remifentanil in human heparinised whole blood by tandem mass spectrometry with short-column separation
| Autor: | Guido C. J. M. Broeders, Jan Dankers, Cees van der Heiden, Jos van den Elshout, Johan Bender, Krzysztof Selinger |
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| Rok vydání: | 1999 |
| Předmět: |
Detection limit
Chromatography Heparin Chemistry Clinical Biochemistry Remifentanil Pharmaceutical Science Tandem mass spectrometry Mass spectrometry Mass Spectrometry Analytical Chemistry Column chromatography Piperidines Pharmacokinetics Calibration Drug Discovery medicine Humans Quantitative analysis (chemistry) Anesthetics Intravenous Spectroscopy medicine.drug Whole blood |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 21:559-567 |
| ISSN: | 0731-7085 |
| DOI: | 10.1016/s0731-7085(99)00151-x |
| Popis: | For the use in pharmacokinetic studies, a fast and sensitive assay method was developed for the determination of remifentanil in human heparinised whole blood samples of 0.5 ml. The assay method is based on tandem mass spectrometry detection (LC-MS/MS). The limit of quantification is 0.1 ng/ml and linear up to 50 ng/ml. The precision, accuracy, recovery and applicability were found to be adequate for pharmacokinetic studies. |
| Databáze: | OpenAIRE |
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