Wet-wrap treatment using dilutions of tacrolimus ointment and fluticasone propionate cream in human APOC1 (+/+) mice with atopic dermatitis

Autor: Arnold P. Oranje, Errol P. Prens, R. Verbeek, I. Haspels, Perry Verzaal, Lex Nagelkerken
Přispěvatelé: Dermatology, Erasmus MC other, TNO Kwaliteit van Leven
Rok vydání: 2009
Předmět:
Allergy
Biomedical Research
Ointments
Atopy
Mice
Fluticasone
drug effect
article
Atopic dermatitis
skin water loss
surgical procedures
operative
priority journal
Models
Animal
histopathology
Disease Progression
disease severity
Apolipoprotein C
medicine.drug
medicine.medical_specialty
Transepidermal water loss
animal experiment
Mice
Transgenic
chemical and pharmacologic phenomena
Dermatology
Tacrolimus
Fluticasone propionate
Mouse model
animal tissue
Dermatitis
Atopic
medicine
Animals
Humans
controlled study
Biology
mouse
Apolipoprotein C-I
nonhuman
Dose-Response Relationship
Drug
Emollients
fluticasone propionate
business.industry
animal model
Wet-wrap treatment
treatment response
apolipoprotein C1
medicine.disease
Bandages
clinical feature
skinfold thickness
Androstadienes
Calcineurin
drug formulation
occlusive dressing
weight reduction
business
Zdroj: British Journal of Dermatology, 160(1), 54-61. Wiley-Blackwell Publishing Ltd
British Journal of Dermatology, 1, 160, 54-61
ISSN: 1365-2133
0007-0963
Popis: Background: Wet-wrap treatment (WWT) with diluted topical steroids is widely used in atopic dermatitis (AD). Mice with transgenic overexpression of human apolipoprotein C1 (APOC1) in the liver and the skin are not only characterized by hyperlipidaemia and raised IgE levels, but also by pruritic dermatitis and a disturbed skin barrier function, providing a novel in vivo mouse model for AD. Objectives: We investigated an adapted WWT method in the AD model in APOC1 mice in order to establish its efficacy. Methods: The effect of topical 0.1% and 0.03% tacrolimus ointment, tacrolimus base ointment, different dilutions of 0.05% fluticasone propionate (FP) cream and emollient on the development of dermatitis in APOC1 mice was investigated. WWT was performed with 0.03% tacrolimus ointment or 0.017% FP cream. Results: AD in APOC1 mice responded to topical treatment with tacrolimus or FP. In contrast to tacrolimus treatment, FP treatment was associated with loss of body weight. WWT reinforced several therapeutic aspects, notably improvements in transepidermal water loss and in epidermal thickness. WWT using tacrolimus 0.03% ointment was more effective than WWT using FP 0.017% cream. Conclusions: AD in APOC1 mice responds to treatment with (diluted) tacrolimus or FP; treatment with FP cream, but not tacrolimus ointment, was associated with weight loss. In this study, the adapted WWT using tacrolimus or FP in mice had a limited improving effect as compared with open application of tacrolimus or FP. © 2008 The Authors.
Databáze: OpenAIRE
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