Quantitative analysis of natural cyclodextrins by high-performance liquid chromatography with pulsed amperometric detection: Application to cell permeation study
| Autor: | Heli Raatikainen, Pekka Keski-Rahkonen, Tarja Toropainen, Marko Lehtonen, Tomi Järvinen, Pekka Jarho |
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| Rok vydání: | 2008 |
| Předmět: |
chemistry.chemical_classification
Cyclodextrins Cell Membrane Permeability Chromatography Cyclodextrin Resolution (mass spectrometry) Clinical Biochemistry Extraction (chemistry) Reproducibility of Results Cell Biology General Medicine Repeatability Reference Standards Permeation Sensitivity and Specificity Biochemistry High-performance liquid chromatography Amperometry Analytical Chemistry chemistry Calibration Electrochemistry Quantitative analysis (chemistry) Chromatography High Pressure Liquid |
| Zdroj: | Journal of Chromatography B. 867:90-98 |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/j.jchromb.2008.03.020 |
| Popis: | Simple HPLC-PAD methods were developed for quantitation of cyclodextrins (CDs) in aqueous matrices from in vitro cell permeation studies. C-18 solid-phase extraction was used for sample pretreatment. Samples were analysed using acetonitrile-water mobile phase with post-column alkalization by 0.5M NaOH. Zorbax SB-Aq (for alpha-CD) and Zorbax SB-Phenyl (for beta-CD and gamma-CD) columns gave excellent peak shape and sufficient resolution of CD to glucose (2.7-3.2). The methods showed good concentration-response relationship (ror = 0.999), precision (RSD% 0.7-5.1), repeatability (RSD% 3.4-13.7) and accuracy (87-107%). The limits of quantitation were 0.78, 0.46 and 0.52 microg/ml for alpha-CD, beta-CD and gamma-CD (RSD% of 10.6, 8.1 and 16.3, respectively). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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