Prevalence and molecular epidemiology of bovine leukemia virus in Colombian cattle
| Autor: | Pilar Moreno, Juan Sebastián Quintero, Diego Ortiz, Alfredo Sánchez, Adriana P. Corredor-Figueroa, Nury N. Olaya-Galán, Sandra Salas, Juan Cristina, Julio Cesar Tobón, Alvaro Fajardo, María Fernanda Gutiérrez, Martín Soñora |
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| Přispěvatelé: | Corredor-Figueroa A. P., Salas S., Olaya-Galán N. N., Quintero J. S., Fajardo Rossi Álvaro, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares, Sóñora Martín, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares, Moreno Karlen María del Pilar, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares, Cristina Juan, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares, Sánchez A., Tobón J., Ortiz D., Gutiérrez M. F. |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Genes Viral animal diseases viruses Envelope gene Polymerase Chain Reaction Agricultural worker Bovine leukemia virus Animal tissue law.invention law Agricultural land Genotype Leukemia Virus Bovine Prevalence Public Health Surveillance Geography Medical Polymerase chain reaction Phylogeny Molecular Epidemiology biology virus diseases Amplicon Phylogeography Infectious Diseases Government Molecular epidemiology Female Microbiology (medical) Livestock 030106 microbiology Blv Colombia Microbiology Article 03 medical and health sciences Genetics Animals Genetic variability Animal experiment Viral genotypes Molecular Biology Allele frequency Genotyping Ecology Evolution Behavior and Systematics Cow Genetic Variation Enzootic Bovine Leukosis biology.organism_classification Nonhuman Virology Gene frequency 030104 developmental biology Enzootic bovine leukosis Cattle Controlled study |
| Zdroj: | Repositorio EdocUR-U. Rosario Universidad del Rosario instacron:Universidad del Rosario COLIBRI Universidad de la República instacron:Universidad de la República |
| Popis: | Bovine leukemia virus (BLV) is one of the five agents considered most significant for cattle. It is important to determine the prevalence and molecular epidemiology of BLV throughout the country in order to gain a more thorough understanding of the current situation of BLV and to reveal the possibility of masked genotypes that the primers used by OIE are unable to identify. Blood samples were collected at random from 289 cows distributed in 75 farms across the country. PCR amplification of env, gag and tax gene segments was performed. The obtained amplicons were sequenced and then subjected to phylogenetic analyses. A total of 62% of the cows present at 92% of the farms were BLV-positive for gag fragment. Genotype 1 was exclusively detected by env gene segment when analyzed using previously reported primers. However, tax gene analysis revealed circulation of genotype 6 variants, which were also detected based on env gene analysis with newly designed primers. These results indicate that current genotyping approaches based on partial env sequencing may bias BLV genetic variability approaches and underestimate the diversity of the detected BLV genotypes. This report is one of the first molecular and epidemiological studies of BLV conducted in Colombia, which contributes to the global epidemiology of the virus; it also highlights the substantial impact of BLV on the country's livestock and thus is a useful resource for farmers and government entities. © 2020 The Authors |
| Databáze: | OpenAIRE |
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