A Novel Marker for Undifferentiated Human Embryonic Stem Cells
| Autor: | Kumiko Kobayashi, Tatsuhiko Arakawa, Asano Kouji, Masaki Yagi, Taro Tachibana, Kiyoshi Higashi, Koichi Saito |
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| Rok vydání: | 2015 |
| Předmět: |
medicine.drug_class
Cellular differentiation Immunology Cell Gene Expression Biology Monoclonal antibody Mice Antigen Cell Line Tumor medicine Animals Humans Immunology and Allergy Induced pluripotent stem cell Embryonic Stem Cells reproductive and urinary physiology Neurons Hybridomas Antibodies Monoclonal Cell Differentiation Epithelial Cells Embryo Mammalian equipment and supplies Embryonic stem cell Molecular biology medicine.anatomical_structure Cell culture Basigin embryonic structures biological phenomena cell phenomena and immunity Biomarkers |
| Zdroj: | Monoclonal Antibodies in Immunodiagnosis and Immunotherapy. 34:7-11 |
| ISSN: | 2167-9436 |
| DOI: | 10.1089/mab.2014.0075 |
| Popis: | Human embryonic stem cells (hESCs) are pluripotent stem cells from early embryos, and their self-renewal capacity depends on the sustained expression of hESC-specific molecules and the suppressed expression of differentiation-associated genes. To discover novel molecules expressed on hESCs, we generated a panel of monoclonal antibodies against undifferentiated hESCs. The antigen recognized by MAb2 is expressed on the cell surface of undifferentiated hESCs; three diffused bands with molecular mass between 30 and 60 kDa in the lysates of hESCs were diminished during hESC differentiation into neural cells. The expression of MAb2 antigen was also observed on the plasma membrane of lung cancer cells, and MAb2 detected 55, 50, and 35 kDa protein bands in the cell lysates. Immunoprecipitation followed by proteomics analyses identified CD147/basigin as a MAb2 antigen. Finally, the positive expression of CD147/basigin protein in undifferentiated hESCs was confirmed. These results suggested that CD147/basigin could be another undifferentiated hESC marker. |
| Databáze: | OpenAIRE |
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