The carbonic anhydrase isoforms of Chlamydomonas reinhardtii: intracellular location, expression, and physiological roles

Autor: James V. Moroney, Katelyn A. Fusilier, Wesley D. Frey, Jing Yang, Robert J. DiMario, Bratati Mukherjee, Yunbing Ma, Trang T. Pham, Tiffany A. Simms
Rok vydání: 2010
Předmět:
Zdroj: Photosynthesis research. 109(1-3)
ISSN: 1573-5079
Popis: Aquatic photosynthetic organisms, such as the green alga Chlamydomonas reinhardtii, respond to low CO2 conditions by inducing a CO2 concentrating mechanism (CCM). Carbonic anhydrases (CAs) are important components of the CCM. CAs are zinc-containing metalloenzymes that catalyze the reversible interconversion of CO2 and HCO3 −. In C. reinhardtii, there are at least 12 genes that encode CA isoforms, including three alpha, six beta, and three gamma or gamma-like CAs. The expression of the three alpha and six beta genes has been measured from cells grown on elevated CO2 (having no active CCM) versus cells growing on low levels of CO2 (with an active CCM) using northern blots, differential hybridization to DNA chips and quantitative RT-PCR. Recent RNA-seq profiles add to our knowledge of the expression of all of the CA genes. In addition, protein content for some of the CA isoforms was estimated using antibodies corresponding to the specific CA isoforms: CAH1/2, CAH3, CAH4/5, CAH6, and CAH7. The intracellular location of each of the CA isoforms was elucidated using immunolocalization and cell fractionation techniques. Combining these results with previous studies using CA mutant strains, we will discuss possible physiological roles of the CA isoforms concentrating on how these CAs might contribute to the acquisition and retention of CO2 in C. reinhardtii.
Databáze: OpenAIRE
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