Genome, Proteome and Structure of a T7-Like Bacteriophage of the Kiwifruit Canker Phytopathogen Pseudomonas syringae pv. actinidiae
| Autor: | Andrew R. Pitman, Rebekah A. Frampton, Peter C. Fineran, Danni Chen, Mihnea Bostina, Brian Tong, Torsten Kleffmann, Vivienne L. Young, Richard A. Easingwood, Corinda Taylor, Elena Lopez Acedo |
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| Rok vydání: | 2015 |
| Předmět: |
canker
Proteome Actinidia lcsh:QR1-502 Pseudomonas syringae cryo-electron microscopy Genome Viral Genome Article lcsh:Microbiology Pseudomonas syringae pv. actinidiae Microbiology Bacteriophage Viral Proteins Podoviridae proteomics bacteriophage kiwifruit Virology Lysogenic cycle genomics medicine Bacteriophages single particle reconstruction Plant Diseases Canker biology medicine.disease biology.organism_classification biocontrol agent Infectious Diseases Lytic cycle Fruit vB_PsyP_phiPsa17 |
| Zdroj: | Viruses Volume 7 Issue 7 Pages 3361-3379 Viruses, Vol 7, Iss 7, Pp 3361-3379 (2015) Viruses; Volume 7; Issue 7; Pages: 3361-3379 |
| ISSN: | 1999-4915 |
| Popis: | Pseudomonas syringae pv. actinidiae is an economically significant pathogen responsible for severe bacterial canker of kiwifruit (Actinidia sp.). Bacteriophages infecting this phytopathogen have potential as biocontrol agents as part of an integrated approach to the management of bacterial canker, and for use as molecular tools to study this bacterium. A variety of bacteriophages were previously isolated that infect P. syringae pv. actinidiae, and their basic properties were characterized to provide a framework for formulation of these phages as biocontrol agents. Here, we have examined in more detail φPsa17, a phage with the capacity to infect a broad range of P. syringae pv. actinidiae strains and the only member of the Podoviridae in this collection. Particle morphology was visualized using cryo-electron microscopy, the genome was sequenced, and its structural proteins were analysed using shotgun proteomics. These studies demonstrated that φPsa17 has a 40,525 bp genome, is a member of the T7likevirus genus and is closely related to the pseudomonad phages φPSA2 and gh-1. Eleven structural proteins (one scaffolding) were detected by proteomics and φPsa17 has a capsid of approximately 60 nm in diameter. No genes indicative of a lysogenic lifecycle were identified, suggesting the phage is obligately lytic. These features indicate that φPsa17 may be suitable for formulation as a biocontrol agent of P. syringae pv. actinidiae. |
| Databáze: | OpenAIRE |
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