MYB43 in Oilseed Rape (Brassica napus) Positively Regulates Vascular Lignification, Plant Morphology and Yield Potential but Negatively Affects Resistance to Sclerotinia sclerotiorum
| Autor: | Jin Xiaoyun, Tan Li, Na Lin, Yuan Chenglong, Chai Yourong, Yin Nengwen, Yufei Xue, Liao Xueli, Jiang Jiayi, Lu Qifeng |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine lcsh:QH426-470 vessel Genetically modified crops Plant disease resistance xylem 01 natural sciences interfascicular fiber oilseed rape (Brassica napus) resistance 03 medical and health sciences chemistry.chemical_compound Genetics Lignin Sieve tube element Genetics (clinical) MYB43 plant morphology biology Sclerotinia sclerotiorum fungi Xylem food and beverages yield potential biology.organism_classification Cell biology lcsh:Genetics 030104 developmental biology chemistry Pith Silique lodging 010606 plant biology & botany |
| Zdroj: | Genes Volume 11 Issue 5 Genes, Vol 11, Iss 581, p 581 (2020) |
| ISSN: | 2073-4425 |
| DOI: | 10.3390/genes11050581 |
| Popis: | Arabidopsis thaliana MYB43 (AtMYB43) is suggested to be involved in cell wall lignification. PtrMYB152, the Populus orthologue of AtMYB43, is a transcriptional activator of lignin biosynthesis and vessel wall deposition. In this research, MYB43 genes from Brassica napus (rapeseed) and its parental species B. rapa and B. oleracea were molecularly characterized, which were dominantly expressed in stem and other vascular organs and showed responsiveness to Sclerotinia sclerotiorum infection. The BnMYB43 family was silenced by RNAi, and the transgenic rapeseed lines showed retardation in growth and development with smaller organs, reduced lodging resistance, fewer silique number and lower yield potential. The thickness of the xylem layer decreased by 28% the numbers of sclerenchymatous cells, vessels, interfascicular fibers, sieve tubes and pith cells in the whole cross section of the stem decreased by 28%, 59%, 48%, 34% and 21% in these lines, respectively. The contents of cellulose and lignin decreased by 17.49% and 16.21% respectively, while the pectin content increased by 71.92% in stems of RNAi lines. When inoculated with S. sclerotiorum, the lesion length was drastically decreased by 52.10% in the stems of transgenic plants compared with WT, implying great increase in disease resistance. Correspondingly, changes in the gene expression patterns of lignin biosynthesis, cellulose biosynthesis, pectin biosynthesis, cell cycle, SA- and JA-signals, and defensive pathways were in accordance with above phenotypic modifications. These results show that BnMYB43, being a growth-defense trade-off participant, positively regulates vascular lignification, plant morphology and yield potential, but negatively affects resistance to S. sclerotiorum. Moreover, this lignification activator influences cell biogenesis of both lignified and non-lignified tissues of the whole vascular organ. |
| Databáze: | OpenAIRE |
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