Glycopeptides in pus of acute pleurisy patients
| Autor: | H. Masuda, T. Ozeki, Yukio Tanaka, Isoko Takazono |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Adult
Male Time Factors Endocrinology Diabetes and Metabolism Ion chromatography Fractionation Biochemistry Chromatography DEAE-Cellulose chemistry.chemical_compound Column chromatography medicine Humans Pleurisy Aged Hexoses Hospital days Chromatography Enzymatic digestion Chemistry Glycopeptides Hexosamines Middle Aged medicine.disease Glycopeptide Sialic acid Pleural Effusion Sialic Acids Female |
| Zdroj: | Biochemical medicine and metabolic biology. 44(3) |
| ISSN: | 0885-4505 |
| Popis: | Glycopeptides were isolated from tryptic digests of pus from acute pleurisy patients. The hexose, hexosamine, and sialic acid contents rose over the first 2–4 days after admission to the hospital, continued at high levels for 5–8 hospital days, and fell to low levels after 9 hospital days. The course of duration after admission to the hospital was divided into three stages: 1–4 days after admission to the hospital, 5–8 hospital days, and 9–21 hospital days. Materials corresponding to these three stages were then collected for fractionation by DEAE-cellulose column chromatography. Fractionation of glycopeptides by DEAE-cellulose column chromatography yielded three glycopeptide fractions at 0.05 to 0.2 m NaCl. Column chromatography of crude material on DEAE-cellulose showed an increase in the 0.2 m NaCl fraction from the concentrate over a period of 4–8 hospital days due to a large increase in sialic acid-rich glycopeptide fraction. |
| Databáze: | OpenAIRE |
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