Magnetic Bead Cellulose as a Suitable Support for Immobilization of α-Chymotrypsin
| Autor: | Zdenka Kučerová, Petr Prikryl, Marie Tichá, Jiri Lenfeld, Daniel Horák |
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| Rok vydání: | 2012 |
| Předmět: |
Immobilized enzyme
Molecular Sequence Data Bioengineering Mass spectrometry Applied Microbiology and Biotechnology Biochemistry chemistry.chemical_compound Desorption Animals Chymotrypsin Humans Viscose Amino Acid Sequence Sulfones Cellulose Molecular Biology Gastric Juice Chromatography biology Proteolytic enzymes General Medicine Enzymes Immobilized Microspheres Pepsin A Solubility chemistry Proteolysis Magnets biology.protein Magnetic nanoparticles Cattle Biotechnology |
| Zdroj: | Applied Biochemistry and Biotechnology. 168:295-305 |
| ISSN: | 1559-0291 0273-2289 |
| DOI: | 10.1007/s12010-012-9772-y |
| Popis: | Magnetic bead cellulose was prepared by a suspension method from the mixture of viscose and magnetite using thermal sol-gel transition and regeneration of cellulose. The prepared magnetic particles after their activation with divinyl sulfone were shown to be suitable magnetic carrier for immobilization of α-chymotrypsin and for its application in proteomic studies. The specific activity of the immobilized proteinase was high; its activity did not change in the course of storage. The following properties of the immobilized proteinase were compared with those of the soluble enzyme: pH and temperature dependence of the activity, self-cleavage activity, and possibility of repeated use. α-Chymotrypsin immobilized to magnetic bead cellulose was used for the proteolytic digestion of porcine pepsin A and human gastric juice and a possibility of direct use of enzyme reaction products for matrix-assisted laser desorption/ionization time of flight mass spectrometry analysis was shown. |
| Databáze: | OpenAIRE |
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