Expression Pattern and Immunoregulatory Roles of Galectin-1 and Galectin-3 in Atopic Dermatitis and Psoriasis

Autor: Mab P. Corrêa, Rebeca D. Correia-Silva, Gisela R. Silva Sasso, Solange C. G. P. D’Ávila, Karin V. Greco, Sonia M. Oliani, Cristiane D. Gil
Přispěvatelé: Universidade Estadual Paulista (UNESP), Universidade Federal de São Paulo (UNIFESP), Faculdade de Medicina de São José Do Rio Preto (FAMERP), University College London (UCL)
Rok vydání: 2022
Předmět:
Zdroj: Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
ISSN: 1573-2576
0360-3997
Popis: Made available in DSpace on 2022-04-29T08:46:20Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-01-01 The pathogenesis of atopic dermatitis (AD) and psoriasis (Ps) overlaps, particularly the activation of the immune response and tissue damage. Here, we evaluated galectin (Gal)-1 and Gal-3 levels, which are beta-galactoside-binding proteins with immunomodulatory functions and examined their effects on human keratinocytes stimulated with either interleukin (IL)-4 or IL-17A. Skin biopsies from AD, Ps, and control patients were evaluated using histological and immunohistochemical analyses. Six studies containing publicly available transcriptome data were individually analyzed using the GEO2R tool to detect Gal-1 and Gal-3 mRNA levels. In vitro, IL-4- or IL-17A-stimulated keratinocytes were treated with or without Gal-1 or Gal-3 to evaluate cytokine release and migration. Our findings showed different patterns of expression for Gal-1 and Gal-3 in AD and Ps skins. Densitometric analysis in skin samples showed a marked increase in the protein Gal-1 levels in Ps epidermis and in both AD and Ps dermis compared to controls. Protein and mRNA Gal-3 levels were downregulated in AD and Ps lesional skin compared with the control samples. In vitro, both galectins addition abrogated the release of IL-8 and RANTES in IL-17-stimulated keratinocytes after 24 h, whereas IL-6 release was downregulated by Gal-3 and Gal-1 in IL-4- and IL-17-stimulated cells, respectively. Administration of both galectins also increased the rate of keratinocyte migration under IL-4 or IL-17 stimulation conditions compared with untreated cells. Altogether, the immunoregulatory and migration effects of Gal-1 and Gal-3 on keratinocytes under inflammatory microenvironment make them interesting targets for future therapies in cutaneous diseases. Graphical abstract: [Figure not available: see fulltext.] Universidade Estadual Paulista (UNESP) Instituto de Biociências Letras E Ciências Exatas Programa de Pós-Graduação Em Biociências, SP Departamento de Morfologia E Genética Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina, Rua Botucatu 740, Ed. Lemos Torres – 3º andar, SP Faculdade de Medicina de São José Do Rio Preto (FAMERP) Departamento de Patologia E Medicina Forense, SP Division of Surgery and Interventional Science The Griffin Institute University College London (UCL) Universidade Estadual Paulista (UNESP) Instituto de Biociências Letras E Ciências Exatas Programa de Pós-Graduação Em Biociências, SP
Databáze: OpenAIRE
Externí odkaz: