Molecular Typing of the Etiologic Agent of Human Granulocytic Ehrlichiosis
| Autor: | Sarah E. Carter, M. Dana Ravyn, Russell C. Johnson, Yaning Xu |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Microbiology (medical)
Ehrlichiosis Chlamydiology and Rickettsiology Ehrlichia Biology HindIII Polymerase Chain Reaction law.invention Dogs law medicine Animals Humans Typing Polymerase chain reaction Genetics medicine.disease biology.organism_classification Virology Bacterial Typing Techniques EcoRV biology.protein Restriction digest Restriction fragment length polymorphism Polymorphism Restriction Fragment Length Bacterial Outer Membrane Proteins |
| Zdroj: | Journal of Clinical Microbiology. 39:3398-3401 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.39.9.3398-3401.2001 |
| Popis: | The p44 gene of the agent of human granulocytic ehrlichiosis (aoHGE) encodes a 44-kDa major outer surface protein. A technique was developed for the typing of the aoHGE based on the PCR amplification of the p44 gene followed by a multiple restriction digest with Hin dIII, Eco RV, and Asp I to generate restriction fragment length polymorphism patterns. Twenty-four samples of the aoHGE were collected from geographically dispersed sites in the United States and included isolates from humans, equines, canines, small mammals, and ticks. Six granulocytic ehrlichiosis (GE) types were identified. The GE typing method is relatively simple to perform, is reproducible, and is able to differentiate among the various isolates of granulocytic ehrlichiae in the United States. These characteristics suggest that this GE typing method may be an important epizootiological and epidemiological tool. |
| Databáze: | OpenAIRE |
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