Olig2 defines a subset of neural stem cells that produce specific olfactory bulb interneuron subtypes in the subventricular zone of adult mice
| Autor: | Ángela del Águila, Mike Adam, Kristy Ullom, Nicholas Shaw, Shenyue Qin, Jacqueline Ehrman, Diana Nardini, Joseph Salomone, Brian Gebelein, Q. Richard Lu, Steven S. Potter, Ronald Waclaw, Kenneth Campbell, Masato Nakafuku |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Male
Mice Knockout Neurogenesis Cell Cycle Oligodendrocyte Transcription Factor 2 Stem Cells and Regeneration Olfactory Bulb nervous system diseases Gene Knockout Techniques Mice nervous system Neural Stem Cells Interneurons Lateral Ventricles Animals Cell Lineage Female biological phenomena cell phenomena and immunity Transcriptome Molecular Biology reproductive and urinary physiology Cells Cultured Developmental Biology Signal Transduction |
| Zdroj: | Development |
| Popis: | Distinct neural stem cells (NSCs) reside in different regions of the subventricular zone (SVZ) and generate multiple olfactory bulb (OB) interneuron subtypes in the adult brain. However, the molecular mechanisms underlying such NSC heterogeneity remain largely unknown. Here, we show that the basic helix-loop-helix transcription factor Olig2 defines a subset of NSCs in the early postnatal and adult SVZ. Olig2-expressing NSCs exist broadly but are most enriched in the ventral SVZ along the dorsoventral axis complementary to dorsally enriched Gsx2-expressing NSCs. Comparisons of Olig2-expressing NSCs from early embryonic to adult stages using single cell transcriptomics reveal stepwise developmental changes in their cell cycle and metabolic properties. Genetic studies further show that cross-repression contributes to the mutually exclusive expression of Olig2 and Gsx2 in NSCs/progenitors during embryogenesis, but that their expression is regulated independently from each other in adult NSCs. Finally, lineage-tracing and conditional inactivation studies demonstrate that Olig2 plays an important role in the specification of OB interneuron subtypes. Altogether, our study demonstrates that Olig2 defines a unique subset of adult NSCs enriched in the ventral aspect of the adult SVZ. |
| Databáze: | OpenAIRE |
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