Detection of coagulase gene in Staphylococcus aureus from several dairy farms in East Java, Indonesia, by polymerase chain reaction
| Autor: | Poedji Hastutiek, Wiwiek Tyasningsih, Mirza Atikah Madarina Hisyam, Mustofa Helmi Effendi |
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| Rok vydání: | 2019 |
| Předmět: |
Staphylococcus aureus
040301 veterinary sciences Veterinary medicine Biology medicine.disease_cause SF1-1100 polymorphism law.invention Microbiology 0403 veterinary science 03 medical and health sciences law Polymorphism (computer science) SF600-1100 Genotype medicine Gene Polymerase chain reaction coagulase gene 0303 health sciences Genetic diversity General Veterinary 030306 microbiology raw milk coagulase test 04 agricultural and veterinary sciences Raw milk Animal culture Coagulase Research Article |
| Zdroj: | Veterinary World, Vol 12, Iss 1, Pp 68-71 (2019) Veterinary World |
| ISSN: | 2231-0916 0972-8988 |
| DOI: | 10.14202/vetworld.2019.68-71 |
| Popis: | Aim: This study was conducted to study the coagulase (coa) gene-based genetic diversity of Staphylococcus aureus, isolated from different samples of cattle from three different regions in East Java Province, Indonesia. Materials and Methods: A total of 160 raw milk samples collected in East Java Province, Indonesia, were screened for the presence of S. aureus. The presumptive isolates were confirmed by coa test. The confirmed S. aureus isolates were subjected to coa gene polymerase chain reaction. Results: Of 160 different samples, 20 (12.5%) isolates of S. aureus were confirmed by positive coa test. Of 20 S. aureus isolates, 19 (95%) isolates carried coa gene. Six different genotypes of coa gene, i.e., 440 bp, 510 bp, 547 bp, 680 bp, 740 bp, and 820 bp were obtained. One coa genotypes, 510 bp (10 isolates) were observed in polymorphism to be more prevalent than the others, and the genotype was present in at least one isolates from every region. Conclusion: It can be concluded that coa gene is easily epidemiological tool for detection of variation strain from S. aureus. Keywords: coagulase gene, coagulase test, polymorphism, raw milk, Staphylococcus aureus |
| Databáze: | OpenAIRE |
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