Detection of Giardia Trophozoites in Archival Pathology Specimens of Human Small Intestine

Autor:	Edward L. Jarroll, Harry van Keulen, Stanley L. Erlandsen, P. Timothy Macechko, Andrew Gurien, Thomas Mulgrew
Rok vydání:	1998
Předmět:	Confocal H&E stain Giardia Biology medicine.disease_cause biology.organism_classification Molecular biology Mucus Small intestine Staining medicine.anatomical_structure Microscopy medicine Giardia lamblia Instrumentation
Zdroj:	Microscopy and Microanalysis. 4:397-403
ISSN:	1435-8115 1431-9276
DOI:	10.1017/s1431927698980370
Popis:	The examination of archival pathology specimens of human small intestine by light microscopy, field emission scanning electron microscopy (FESEM), and confocal scanning laser microscopy using fluorescent in situ hybridization (FISH) techniques was undertaken to better understand the epidemiology of Giardia. Giardia trophozoites were tentatively identified in the light microscope after hematoxylin and eosin (H&E) staining. The organisms were adherent to the intestinal epithelium where they were also associated with strands of mucus within the lumen. Fluorochrome-conjugated antisense oligonucleotide probes, developed for the 16S rRNA of Giardia lamblia and Giardia muris, were used in FISH experiments with confocal scanning laser microscopy. Positive identification of trophozoites could be obtained with the G. lamblia probe, but not with the G. muris probe. FESEM examination of serial sections adjacent to FISH-stained sections revealed trophozoites characterized by their morphological features. The 16S rDNA probes specifically distinguished different species of Giardia, but whether multiple infections can occur within an individual host could not be determined.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ef34657715c689f9f61a276a3daf2c95 https://doi.org/10.1017/s1431927698980370 Zobrazit plný text záznamu