Metabolic-cytokine responses to a second immunological challenge with LPS in mice withT. gondiiinfection
| Autor: | Hernan R. Chang, Lucien Girardier, Irène Garcia, Rudolf Lucas, Abdul G. Dulloo, Denis Arsenijevic, Jean-Claude Pechère, Josiane Seydoux |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Lipopolysaccharides
Physiology Ratón Endocrinology Diabetes and Metabolism medicine.medical_treatment RNA Messenger/metabolism Tumor Necrosis Factor-alpha/biosynthesis Anorexia ddc:616.07 Spleen/metabolism Biology Blood–brain barrier Cachexia Mice Physiology (medical) Gene expression medicine Animals Brain/metabolism RNA Messenger Tumor Necrosis Factor-alpha Interleukin-4/biosynthesis Cytokines/*blood/genetics Lipopolysaccharides/*immunology/pharmacology Brain Toxoplasmosis Animal/immunology/*metabolism Interleukin-10/biosynthesis medicine.disease Toxoplasmosis Interleukin-10 Toxoplasmosis Animal medicine.anatomical_structure Cytokine Blood-Brain Barrier Chronic Disease Immunology Cytokines Female Interleukin-4 medicine.symptom Energy Metabolism Toxoplasma Thermogenesis Spleen |
| Zdroj: | American Journal of Physiology, Vol. 274, No 3 Pt 1 (1998) pp. E439-445 Europe PubMed Central Scopus-Elsevier |
| ISSN: | 1522-1555 0193-1849 0002-9513 |
| DOI: | 10.1152/ajpendo.1998.274.3.e439 |
| Popis: | Injection of 10 cysts of Toxoplasma gondii (Me49 strain) into Swiss Webster mice results in 1) an acute phase of infection lasting for 2–3 wk, characterized by weight loss, and 2) a chronic phase in which surviving mice show either partial weight recovery (Gainers) or persistent, although stable, cachexia (Nongainers). In response to a second immunological stimulation with lipopolysaccharide (LPS) in the chronic phase of the infection, it is shown that 1) the increase in energy expenditure was more prolonged in both groups of infected mice than in controls, 2) the intensity and duration of hypophagia were also differently affected with Nongainers > Gainers > controls, and 3) the infected mice had higher serum levels of tumor necrosis factor-α (TNF-α) and interleukin (IL)-10 and a lower ratio of IL-10 to TNF-α than controls. In contrast, serum IL-4 increased to the same level in all three groups. Evaluation of the permeability of the blood-brain barrier by intravenous injection of Evans blue revealed a marked staining in the brain of only the infected Nongainers. Taken together, these results indicate that, in mice with chronic toxoplasmosis, a second nonspecific challenge (with LPS) exacerbates the hypophagic and hypermetabolic states, the latter being associated with hyperresponsiveness in TNF-α and IL-10 production. Furthermore, the greater exacerbation of the hypophagic state in mice showing persistent cachexia may be due to a preexisting higher permeability of the blood-brain barrier, which would allow a greater access of plasma-borne cytokines and/or other neuroimmunologically active substances to the central nervous system. |
| Databáze: | OpenAIRE |
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