Continuous (R)-mandelic acid production in an enzyme membrane reactor
| Autor: | Dj. Vasic-Racki, M.-R. Kula, M. Jonas, C. Wandrey, Werner Hummel |
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| Jazyk: | angličtina |
| Rok vydání: | 1989 |
| Předmět: |
chemistry.chemical_classification
Phenylglyoxylic acid Chromatography biology Membrane reactor (R)-mandelic acid enzyme membrane reactor mathematical model Dehydrogenase General Medicine Mandelic acid Formate dehydrogenase Applied Microbiology and Biotechnology Cofactor chemistry.chemical_compound Enzyme chemistry biology.protein Enzyme kinetics Biotechnology |
| Popis: | The continuous conversion of phenylglyoxylic acid to (R)-mandelic acid was performed in an enzyme membrane reactor with simultaneous coenzyme regeneration employing mandelate dehydrogenase and formate dehydrogenase. A mathematical model of the coupled enzyme reactions was formulated and applied to determine optimal conditions for (R)-mandelic acid production. The experiments and calculations showed that the optimal operational points depend strongly on enzyme kinetics. The individual kinetic parameters determined were appropriate for the description of (R)-mandelic acid production in the simultaneous two-enzyme process. Space time yields of 700 g/(l· day) were obtained at low enzyme consumption. |
| Databáze: | OpenAIRE |
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