Rev-Erb modulates retinal visual processing and behavioral responses to light

Autor: Ouafa Ait-Hmyed Hakkari, Marie-Paule Felder-Schmittbuhl, Mohammed Bennis, Elise Savier, Jorge E. Mendoza, David Hicks, Perrine Spinnhirny, Niyazi Acar
Přispěvatelé: Institut des Neurosciences Cellulaires et Intégratives (INCI), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Pharmacology, Neurobiology and Comportement Laboratory, Department of Biology, Sciences Faculty, Université Cadi Ayyad [Marrakech] (UCA), Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Centre National de la Recherche Scientifique (CNRS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), Équipe 'Rythme, vie et mort de la rétine', Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Retina France, Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Institut des Neurosciences Cellulaires et Intégratives ( INCI ), Université de Strasbourg ( UNISTRA ) -Centre National de la Recherche Scientifique ( CNRS ), Université Cadi Ayyad [Marrakech], Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Université de Strasbourg ( UNISTRA ) -Institut des Neurosciences Cellulaires et Intégratives (INCI)-Centre National de la Recherche Scientifique ( CNRS ), Université de Strasbourg (UNISTRA)-Institut des Neurosciences Cellulaires et Intégratives (INCI)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Retinal Ganglion Cells
Light
[SDV]Life Sciences [q-bio]
Circadian clock
electroretinogram
Biochemistry
chemistry.chemical_compound
0302 clinical medicine
circadian clock
skin and connective tissue diseases
ComputingMilieux_MISCELLANEOUS
Mice
Knockout
ipRGCs
Behavior
Animal
photoreceptors
organization
Circadian Rhythm
medicine.anatomical_structure
rod
transcription
Biotechnology
Photopic vision
Melanopsin
negative masking
rat retina
Biology
Retina
03 medical and health sciences
[ SDV.MHEP ] Life Sciences [q-bio]/Human health and pathology
Circadian Clocks
Genetics
medicine
Animals
Circadian rhythm
Scotopic vision
melanopsin-knockout mice
Molecular Biology
mouse
Intrinsically photosensitive retinal ganglion cells
Rod Opsins
Retinal
ganglion-cells
body regions
mammalian retina
030104 developmental biology
chemistry
Nuclear Receptor Subfamily 1
Group D
Member 1
sense organs
Neuroscience
030217 neurology & neurosurgery
Photic Stimulation
[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Zdroj: FASEB Journal
FASEB Journal, Federation of American Society of Experimental Biology, 2016, 30 (11), pp.3690-3701. ⟨10.1096/fj.201600414R⟩
FASEB Journal, Federation of American Society of Experimental Biology, 2016, 30 (11), pp.3690-3701. 〈http://www.fasebj.org/content/30/11/3690.short〉. 〈10.1096/fj.201600414R〉
ISSN: 0892-6638
1530-6860
Popis: International audience; The circadian clock is thought to adjust retinal sensitivity to ambient light levels, yet the involvement of specific clock genes is poorly understood. We explored the potential role of the nuclear receptor subfamily 1, group D, member 1 (REV-ERB; or NR1D1) in this respect. In light-evoked behavioral tests, compared with wild-type littermates, Rev-Erb(-/-) mice showed enhanced negative masking at low light levels (0.1 lx). Rev-Erb(-/-) mouse retinas displayed significantly higher numbers of intrinsically photosensitive retinal ganglion cells (ipRGCs; 62% more compared with wild-type) and more intense melanopsin immunostaining of individual ipRGCs. In agreement with a pivotal role for melanopsin, negative masking at low light intensities was abolished in Rev-Erb(-/-)Opn4(-/-) (melanopsin gene) double-null mice. Rev-Erb(-/-) mice showed shortened latencies of both a and b electroretinogram waves, modified scotopic and photopic b-wave and scotopic threshold responses, and increased pupillary constriction, all of which suggested increased light sensitivity. However, wild-type and Rev-Erb(-/-) mice displayed no detectable differences by in vivo fundus imaging, retinal histology, or expression of cell type-specific markers for major retinal cell populations. We conclude that REV-ERB plays a major role in retinal information processing, and we speculate that REV-ERB and melanopsin set sensitivity levels of the rod-mediated ipRGC pathway to coordinate activity with ambient light.Ait-Hmyed Hakkari, O., Acar, N., Savier, E., Spinnhirny, P., Bennis, M., Felder-Schmittbuhl, M.-P., Mendoza, J., Hicks, D. Rev-Erb modulates retinal visual processing and behavioral responses to light.
Databáze: OpenAIRE
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