Rev-Erb modulates retinal visual processing and behavioral responses to light
Autor: | Ouafa Ait-Hmyed Hakkari, Marie-Paule Felder-Schmittbuhl, Mohammed Bennis, Elise Savier, Jorge E. Mendoza, David Hicks, Perrine Spinnhirny, Niyazi Acar |
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Přispěvatelé: | Institut des Neurosciences Cellulaires et Intégratives (INCI), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Pharmacology, Neurobiology and Comportement Laboratory, Department of Biology, Sciences Faculty, Université Cadi Ayyad [Marrakech] (UCA), Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Centre National de la Recherche Scientifique (CNRS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), Équipe 'Rythme, vie et mort de la rétine', Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Retina France, Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Institut des Neurosciences Cellulaires et Intégratives ( INCI ), Université de Strasbourg ( UNISTRA ) -Centre National de la Recherche Scientifique ( CNRS ), Université Cadi Ayyad [Marrakech], Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Université de Strasbourg ( UNISTRA ) -Institut des Neurosciences Cellulaires et Intégratives (INCI)-Centre National de la Recherche Scientifique ( CNRS ), Université de Strasbourg (UNISTRA)-Institut des Neurosciences Cellulaires et Intégratives (INCI)-Centre National de la Recherche Scientifique (CNRS) |
Jazyk: | angličtina |
Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Retinal Ganglion Cells Light [SDV]Life Sciences [q-bio] Circadian clock electroretinogram Biochemistry chemistry.chemical_compound 0302 clinical medicine circadian clock skin and connective tissue diseases ComputingMilieux_MISCELLANEOUS Mice Knockout ipRGCs Behavior Animal photoreceptors organization Circadian Rhythm medicine.anatomical_structure rod transcription Biotechnology Photopic vision Melanopsin negative masking rat retina Biology Retina 03 medical and health sciences [ SDV.MHEP ] Life Sciences [q-bio]/Human health and pathology Circadian Clocks Genetics medicine Animals Circadian rhythm Scotopic vision melanopsin-knockout mice Molecular Biology mouse Intrinsically photosensitive retinal ganglion cells Rod Opsins Retinal ganglion-cells body regions mammalian retina 030104 developmental biology chemistry Nuclear Receptor Subfamily 1 Group D Member 1 sense organs Neuroscience 030217 neurology & neurosurgery Photic Stimulation [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology |
Zdroj: | FASEB Journal FASEB Journal, Federation of American Society of Experimental Biology, 2016, 30 (11), pp.3690-3701. ⟨10.1096/fj.201600414R⟩ FASEB Journal, Federation of American Society of Experimental Biology, 2016, 30 (11), pp.3690-3701. 〈http://www.fasebj.org/content/30/11/3690.short〉. 〈10.1096/fj.201600414R〉 |
ISSN: | 0892-6638 1530-6860 |
Popis: | International audience; The circadian clock is thought to adjust retinal sensitivity to ambient light levels, yet the involvement of specific clock genes is poorly understood. We explored the potential role of the nuclear receptor subfamily 1, group D, member 1 (REV-ERB; or NR1D1) in this respect. In light-evoked behavioral tests, compared with wild-type littermates, Rev-Erb(-/-) mice showed enhanced negative masking at low light levels (0.1 lx). Rev-Erb(-/-) mouse retinas displayed significantly higher numbers of intrinsically photosensitive retinal ganglion cells (ipRGCs; 62% more compared with wild-type) and more intense melanopsin immunostaining of individual ipRGCs. In agreement with a pivotal role for melanopsin, negative masking at low light intensities was abolished in Rev-Erb(-/-)Opn4(-/-) (melanopsin gene) double-null mice. Rev-Erb(-/-) mice showed shortened latencies of both a and b electroretinogram waves, modified scotopic and photopic b-wave and scotopic threshold responses, and increased pupillary constriction, all of which suggested increased light sensitivity. However, wild-type and Rev-Erb(-/-) mice displayed no detectable differences by in vivo fundus imaging, retinal histology, or expression of cell type-specific markers for major retinal cell populations. We conclude that REV-ERB plays a major role in retinal information processing, and we speculate that REV-ERB and melanopsin set sensitivity levels of the rod-mediated ipRGC pathway to coordinate activity with ambient light.Ait-Hmyed Hakkari, O., Acar, N., Savier, E., Spinnhirny, P., Bennis, M., Felder-Schmittbuhl, M.-P., Mendoza, J., Hicks, D. Rev-Erb modulates retinal visual processing and behavioral responses to light. |
Databáze: | OpenAIRE |
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