A yeast-based assay identifies drugs active against human mitochondrial disorders
| Autor: | Jean-Paul di Rago, Lars M. Steinmetz, Raeka S. Aiyar, Marie Le Cann, Nahia Ezkurdia, Roza Kucharczyk, Flavie Soubigou, Robert P. St.Onge, Marc Blondel, Bénédicte Salin, Julien Gagneur, Anna Magdalena Kabala, Elodie Couplan |
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| Přispěvatelé: | Grellety, Marie-Lise |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Mitochondrial DNA
Mitochondrial disease Drug Evaluation Preclinical Oxidative phosphorylation Biology medicine.disease_cause Mitochondrial Proton-Translocating ATPases Cell Line Mitochondrial myopathy Drug Discovery medicine Humans [SDV.BC] Life Sciences [q-bio]/Cellular Biology ComputingMilieux_MISCELLANEOUS Mutation Multidisciplinary ATP synthase Gene Expression Profiling Chlorhexidine Mitochondrial Myopathies Biological Sciences medicine.disease Yeast Biochemistry Saccharomycetales Mutagenesis Site-Directed biology.protein Retinitis Pigmentosa Oleic Acid |
| Popis: | Due to the lack of relevant animal models, development of effective treatments for human mitochondrial diseases has been limited. Here we establish a rapid, yeast-based assay to screen for drugs active against human inherited mitochondrial diseases affecting ATP synthase, in particular NARP (neuropathy, ataxia, and retinitis pigmentosa) syndrome. This method is based on the conservation of mitochondrial function from yeast to human, on the unique ability of yeast to survive without production of ATP by oxidative phosphorylation, and on the amenability of the yeast mitochondrial genome to site-directed mutagenesis. Our method identifies chlorhexidine by screening a chemical library and oleate through a candidate approach. We show that these molecules rescue a number of phenotypes resulting from mutations affecting ATP synthase in yeast. These compounds are also active on human cybrid cells derived from NARP patients. These results validate our method as an effective high-throughput screening approach to identify drugs active in the treatment of human ATP synthase disorders and suggest that this type of method could be applied to other mitochondrial diseases. |
| Databáze: | OpenAIRE |
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