Identification and Characterization of Major Constituents in Different-Colored Rapeseed Petals by UPLC–HESI-MS/MS
| Autor: | Ledong Jia, Yin Jiaming, Nengwen Yin, Kun Lu, Cunmin Qu, Jiana Li, Bao-Jin Zhou, Meichen Zhu, Jing Yang, Rui Wang, Shuxian Wang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Rapeseed Coumaric Acids Brassica Color Flowers 01 natural sciences Ferulic acid chemistry.chemical_compound Tandem Mass Spectrometry Hydroxybenzoates Food science Kaempferols Chromatography High Pressure Liquid Isorhamnetin Flavonoids chemistry.chemical_classification biology Plant Extracts Brassica napus 010401 analytical chemistry General Chemistry Hydroxycinnamic acid biology.organism_classification 0104 chemical sciences chemistry Petal General Agricultural and Biological Sciences Kaempferol Quercetin 010606 plant biology & botany |
| Zdroj: | Journal of Agricultural and Food Chemistry. 67:11053-11065 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/acs.jafc.9b05046 |
| Popis: | Oilseed rape (Brassica napus L.) is the second highest yielding oil crop worldwide. In addition to being used as an edible oil and a feed for livestock, rapeseed has high ornamental value. In this study, we identified and characterized the main floral major constituents, including phenolic acids and flavonoids components, in rapeseed accessions with different-colored petals. A total of 144 constituents were identified using ultrahigh-performance liquid chromatography-HESI-mass spectrometry (UPLC-HESI-MS/MS), 57 of which were confirmed and quantified using known standards and mainly contained phenolic acids, flavonoids, and glucosinolates compounds. Most of the epicatechin, quercetin, and isorhamnetin derivates were found in red and pink petals of B. napus, while kaempferol derivates were in yellow and pale white petals. Moreover, petal-specific compounds, including a putative hydroxycinnamic acid derivative, sinapoyl malate, 1-O-sinapoyl-β-d-glucose, feruloyl glucose, naringenin-7-O-glucoside, cyanidin-3-glucoside, cyanidin-3,5-di-O-glucoside, petunidin-3-O-β-glucopyranoside, isorhamnetin-3-O-glucoside, kaempferol-3-O-glucoside-7-O-glucoside, quercetin-3,4'-O-di-β-glucopyranoside, quercetin-3-O-glucoside, and delphinidin-3-O-glucoside, might contribute to a variety of petal colors in B. napus. In addition, bound phenolics were tentatively identified and contained three abundant compounds (p-coumaric acid, ferulic acid, and 8-O-4'-diferulic acid). These results provide insight into the molecular mechanisms underlying petal color and suggest strategies for breeding rapeseed with a specific petal color in the future. |
| Databáze: | OpenAIRE |
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