New group of transmembrane proteins associated with desiccation tolerance in the anhydrobiotic midge Polypedilum vanderplanki
| Autor: | Alexander Nesmelov, Sabina A Kondratyeva, Richard Cornette, Taisiya A Voronina, Elena Shagimardanova, Oleg Gusev, Shoko Tokumoto, Yugo Miyata, Takahiro Kikawada, Ruslan Deviatiiarov |
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| Rok vydání: | 2020 |
| Předmět: |
Molecular biology
Science Amino Acid Motifs Biology Genome Article Chironomidae Cell Line Desiccation tolerance Protein Domains Animals Cluster Analysis Computer Simulation RNA-Seq Cloning Molecular Cryptobiosis Gene Phylogeny chemistry.chemical_classification Genetics Multidisciplinary Dehydration Polypedilum vanderplanki Gene Expression Profiling Cell Membrane Membrane Proteins biology.organism_classification Transmembrane protein Amino acid Transmembrane domain Gene Expression Regulation chemistry Larva Multigene Family Medicine Insect Proteins Gene expression |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-020-68330-6 |
| Popis: | Larvae of the sleeping chironomid Polypedilum vanderplanki are known for their extraordinary ability to survive complete desiccation in an ametabolic state called “anhydrobiosis”. The unique feature of P. vanderplanki genome is the presence of expanded gene clusters associated with anhydrobiosis. While several such clusters represent orthologues of known genes, there is a distinct set of genes unique for P. vanderplanki. These include Lea-Island-Located (LIL) genes with no known orthologues except two of LEA genes of P. vanderplanki, PvLea1 and PvLea3. However, PvLIL proteins lack typical features of LEA such as the state of intrinsic disorder, hydrophilicity and characteristic LEA_4 motif. They possess four to five transmembrane domains each and we confirmed membrane targeting for three PvLILs. Conserved amino acids in PvLIL are located in transmembrane domains or nearby. PvLEA1 and PvLEA3 proteins are chimeras combining LEA-like parts and transmembrane domains, shared with PvLIL proteins. We have found that PvLil genes are highly upregulated during anhydrobiosis induction both in larvae of P. vanderplanki and P. vanderplanki-derived cultured cell line, Pv11. Thus, PvLil are a new intriguing group of genes that are likely to be associated with anhydrobiosis due to their common origin with some LEA genes and their induction during anhydrobiosis. |
| Databáze: | OpenAIRE |
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