Desired and side effects of the supplementation with l -glutamine and l -glutathione in enteric glia of diabetic rats
| Autor: | David R. Linden, Camila Caviquioli Sehaber, Catchia Hermes-Uliana, Cynthia Priscilla do Nascimento Bonato Panizzon, Aline Rosa Trevizan, Jacqueline Nelisis Zanoni, Renata Virginia Fernandes Pereira, Marcílio Hubner de Miranda Neto |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Male
medicine.medical_specialty Histology Glutamine Cell Myenteric Plexus Ileum Diabetes Mellitus Experimental 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Diabetic Neuropathies Diabetes mellitus Internal medicine medicine Animals Rats Wistar Neurons Enteric neuropathy business.industry Cell Biology General Medicine Glutathione medicine.disease Disease Models Animal Endocrinology medicine.anatomical_structure chemistry Dietary Supplements Immunohistochemistry 030211 gastroenterology & hepatology Enteric nervous system business Neuroglia 030217 neurology & neurosurgery |
| Zdroj: | Acta Histochemica. 118:625-631 |
| ISSN: | 0065-1281 |
| Popis: | Background/Aims Enteric neuropathy associated with Diabetes Mellitus causes dysfunction in the digestive system, such as: nausea, diarrhea, constipation, vomiting, among others. The aim of this study was to compare the effects of supplementation with 2% l -glutamine and 1% l -glutathione on neurons and enteric glial cells of ileum of diabetic rats. Methods Thirty male Wistar rats have been used according to these group distributions: Normoglycemic (N), Normoglycemic supplemented with l -glutamine (NG), Normoglycemic supplemented with l -glutathione (NGO), Diabetic (D), Diabetic supplemented with l -glutamine (DG) and Diabetic supplemented with l -glutathione (DGO). After 120 days, the ileum was processed for immunohistochemistry of HuC/D and S100β. Quantitative and morphometric analysis have been performed. Results Diabetic rats presented a decrease in the number of neurons when compared to normoglycemic animals. However, diabetes was not associated with a change in glial density. l -Glutathione prevented the neuronal death in diabetic rats. l -Glutathione increased a glial proliferation in diabetic rats. The neuronal area in diabetic rats increased in relation to the normoglycemics. The diabetic rats supplemented with l -glutamine and l -glutathione showed a smaller neuronal area in comparison to diabetic group. The glial cell area was a decreased in the diabetics. The diabetic rats supplemented with l -glutamine and l -glutathione did not have significant difference in the glial cell body area when compared to diabetic rats. Conclusion It is concluded that the usage of l -glutamine and l -glutathione as supplements presents both desired and side effects that are different for the same substance in considering normoglycemic or diabetic animals. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect