Effect of acute nitrate and citrulline supplementation on muscle microvascular response to ischemia-reperfusion in healthy humans
| Autor: | Stéphane Doutreleau, Thibault Le Roux-Mallouf, Samuel Verges, Florence Vibert |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Adult
Male medicine.medical_specialty Physiology Endocrinology Diabetes and Metabolism Ischemia Vasodilation Hyperemia 030204 cardiovascular system & hematology Plant Roots Nitric oxide 03 medical and health sciences chemistry.chemical_compound Hemoglobins Young Adult 0302 clinical medicine Nitrate Physiology (medical) Internal medicine Oxyde nitrique Citrulline Medicine Humans Single-Blind Method No production Muscle Skeletal Leg Nutrition and Dietetics Nitrates Spectroscopy Near-Infrared Vasomotor business.industry General Medicine medicine.disease Fruit and Vegetable Juices Endocrinology chemistry Biochemistry Oxyhemoglobins Reperfusion Injury Dietary Supplements Microvessels Female Vascular Resistance Beta vulgaris business 030217 neurology & neurosurgery |
| Zdroj: | Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme. 42(9) |
| ISSN: | 1715-5320 |
| Popis: | Nitric oxide (NO) is implicated in vasomotor control mechanisms altering the diameter of the vessels under various physiological and pathological conditions. There are 2 main NO production pathways, 1 NO synthase (NOS) independent (nitrate–nitrite–NO) and the other is NOS dependent (citrulline–arginine–NO). The objective of the study was to evaluate the effect of acute nitrate and citrulline supplementation on post-ischemic vascular response in healthy subjects. Fourteen subjects performed 2-leg vascular occlusion tests, 3 days apart. They were randomly assigned to consume a drink containing 1200 mg (19.4 mmol) of nitrate and 6 g of citrulline (N+C) or a placebo (Pl). Changes in total hemoglobin (Hbtot) and oxyhemoglobin (HbO2) concentrations were recorded by near-infrared spectroscopy on the thigh and calf muscles. No differences between N+C and Pl were observed during the ischemic period. Hbtot increased to a larger extent during the reperfusion period for the thigh (e.g., area under the curve, 821 ± 324 vs. 627 ± 381 mmol·s−1, p = 0.003) and the calf (515 ± 285 vs. 400 ± 275 mmol·s−1, p = 0.029) in the N+C versus Pl conditions. Similar results were found regarding HbO2 for the thigh (e.g., area under the curve, 842 ± 502 vs. 770 ± 491 mmol·s−1, p = 0.077) and the calf (968 ± 536 vs. 865 ± 275 mmol·s−1, p = 0.075). The larger postocclusive Hbtot and HbO2 responses observed after N+C intake suggests a greater post-ischemic vasodilation, which may be due to increased NO availability, via the activation of the 2 main NO production pathways. |
| Databáze: | OpenAIRE |
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