Sugarcane mosaic virus infection of model plants Brachypodium distachyon and Nicotiana benthamiana
| Autor: | Lei Peng, Yang Yongqing, Guangyuan Cheng, Deng Yuqing, Qian Xu, Meng Dong, Zhai Yushan, Jing-sheng Xu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Agriculture (General) Nicotiana benthamiana Plant Science 01 natural sciences Biochemistry S1-972 Food Animals sugarcane Pathogen Infectivity Brachypodium distachyon Ecology biology Potyviridae Inoculation fungi Potyvirus food and beverages 04 agricultural and veterinary sciences biology.organism_classification Virology infection Sugarcane mosaic virus 040103 agronomy & agriculture 0401 agriculture forestry and fisheries Animal Science and Zoology SCMV Agronomy and Crop Science 010606 plant biology & botany Food Science |
| Zdroj: | Journal of Integrative Agriculture, Vol 18, Iss 10, Pp 2294-2301 (2019) |
| ISSN: | 2095-3119 |
| Popis: | Sugarcane mosaic virus (SCMV; genus Potyvirus, family Potyviridae) is a causal pathogen of sugarcane mosaic disease, and it is widespread in regions where sugarcane (Saccharum spp. hybrids) is grown. It is difficult to investigate the molecular mechanism of pathogen infection in sugarcane because of limited genomic information. Here, we demonstrated that SCMV strain FZ1 can systemically infect Brachypodium distachyon inbred line Bd21 and Nicotiana benthamiana through inoculation, double antibody sandwich enzyme-linked immunosorbent, transmission electron microscopy, and reverse transcription PCR assays. The leaves of Bd21 developed mosaic symptoms, while the leaves of N. benthamiana showed no obvious symptoms under the challenge of SCMV-FZ1. We concluded that B. distachyon inbred line Bd21 is a promising experimental model plant compared with N. benthamiana for study on the infectivity of SCMV. This is the first report on the SCMV infection of model plants B. distachyon inbred line Bd21 and N. benthamiana, which will shed light on the mechanism of SCMV infection of sugarcane and benefit sugarcane breeding against sugarcane mosaic disease. |
| Databáze: | OpenAIRE |
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