Connexin 26 Expression in Mammalian Cardiomyocytes
| Autor: | Letizia Mattii, Luca Botta, Manuela Cabiati, Silvia Burchielli, M.A. Morales, Francesca Vaglini, Alessandra Falleni, Francesco Bianchi, S. Del Ry, Antonietta Raffaella Maria Sabbatini, Stefania Moscato |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Swine Connexin lcsh:Medicine Biology 03 medical and health sciences otorhinolaryngologic diseases Myocyte Animals Humans Myocytes Cardiac RNA Messenger Rats Wistar lcsh:Science Regulation of gene expression Multidisciplinary lcsh:R RNA Heart Phenotype Cell biology Rats Connexin 26 030104 developmental biology Gene Expression Regulation Cytoplasm Membrane channel Immunohistochemistry lcsh:Q |
| Zdroj: | Scientific Reports, Vol 8, Iss 1, Pp 1-11 (2018) |
| Popis: | Connexins are a family of membrane-spanning proteins named according to their molecular weight. They are known to form membrane channels mediating cell-cell communication, which play an essential role in the propagation of electrical activity in the heart. Cx26 has been described in a number of tissues but not in the heart, and its mutations are frequently associated with deafness and skin diseases. The aim of this study was to assess the possible Cx26 expression in heart tissues of different mammalian species and to demonstrate its localization at level of cardiomyocytes. Samples of pig, human and rat heart and H9c2 cells were used for our research. Immunohistochemical and molecular biology techniques were employed to test the expression of Cx26. Interestingly, this connexin was found in cardiomyocytes, at level of clusters scattered over the cell cytoplasm but not at level of the intercalated discs where the other cardiac connexins are usually located. Furthermore, the expression of Cx26 in H9c2 myoblast cells increased when they were differentiated into cardiac-like phenotype. To our knowledge, the expression of Cx26 in pig, human and rat has been demonstrated for the first time in the present paper. |
| Databáze: | OpenAIRE |
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