The origin of a morphologically unidentifiable human supernumerary minichromosome traced through sorting, molecular cloning, and in situ hybridisation
| Autor: | L. De Carli, Vittorio Sgaramella, Luca Ferretti, B. D. Young, Elena Raimondi |
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| Rok vydání: | 1991 |
| Předmět: |
medicine.medical_specialty
Centromere Chromosome Disorders Chromosome 9 Cell Separation Biology Molecular cloning Nucleic acid thermodynamics Minichromosome Genetics medicine Humans Abnormalities Multiple Cloning Molecular Genetics (clinical) Chromosome Aberrations Cloning Hybridization probe Infant Newborn Cytogenetics Chromosome Mapping Nucleic Acid Hybridization DNA Flow Cytometry Molecular biology Female Chromosomes Human Pair 9 DNA Probes Research Article |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1468-6244 |
| DOI: | 10.1136/jmg.28.2.92 |
| Popis: | A supernumerary minichromosome has been detected in a severely malformed patient. Attempts at identifying the marker by conventional approaches were unsuccessful. The physical isolation of the minichromosome by fluorescence activated sorting, molecular cloning of its DNA, and in situ hybridisation experiments performed with single copy DNA probes allowed us to show that it was derived from a rearrangement involving the centromere and the proximal region of the short arm of chromosome 9. |
| Databáze: | OpenAIRE |
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