Automating cell detection and classification in human brain fluorescent microscopy images using dictionary learning and sparse coding
| Autor: | Helmut Heinsen, Maryana Alegro, Patricia A. Castruita, Lea T. Grinberg, William W. Seeley, Austin Nguy, Daniela Ushizima, Panagiotis Theofilas |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Computer science Fluorescent Antibody Technique Cell Count Article Pattern Recognition Automated Machine Learning 03 medical and health sciences 0302 clinical medicine Alzheimer Disease Image Processing Computer-Assisted medicine Humans Segmentation Computer vision Reproducibility business.industry General Neuroscience Brain Reproducibility of Results Image segmentation Human brain Autofluorescence 030104 developmental biology medicine.anatomical_structure Microscopy Fluorescence False positive rate Artificial intelligence F1 score Neural coding business 030217 neurology & neurosurgery |
| Zdroj: | Journal of Neuroscience Methods. 282:20-33 |
| ISSN: | 0165-0270 |
| Popis: | Background Immunofluorescence (IF) plays a major role in quantifying protein expression in situ and understanding cell function. It is widely applied in assessing disease mechanisms and in drug discovery research. Automation of IF analysis can transform studies using experimental cell models. However, IF analysis of postmortem human tissue relies mostly on manual interaction, often subjected to low-throughput and prone to error, leading to low inter and intra-observer reproducibility. Human postmortem brain samples challenges neuroscientists because of the high level of autofluorescence caused by accumulation of lipofuscin pigment during aging, hindering systematic analyses. We propose a method for automating cell counting and classification in IF microscopy of human postmortem brains. Our algorithm speeds up the quantification task while improving reproducibility. New method Dictionary learning and sparse coding allow for constructing improved cell representations using IF images. These models are input for detection and segmentation methods. Classification occurs by means of color distances between cells and a learned set. Results Our method successfully detected and classified cells in 49 human brain images. We evaluated our results regarding true positive, false positive, false negative, precision, recall, false positive rate and F1 score metrics. We also measured user-experience and time saved compared to manual countings. Comparison with existing methods We compared our results to four open-access IF-based cell-counting tools available in the literature. Our method showed improved accuracy for all data samples. Conclusion The proposed method satisfactorily detects and classifies cells from human postmortem brain IF images, with potential to be generalized for applications in other counting tasks. |
| Databáze: | OpenAIRE |
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