Human papillomavirus and Epstein-Barr virus in nasopharyngeal carcinoma in a non-endemic eastern european population
Autor: | Jan Laco, Marián Švajdler, Michal Michal, Ondrej Ondič, Lubomir Straka, Roman Mezencev, Alena Skálová, Jana Kašpírková, Dubinsky P, Torday T |
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Rok vydání: | 2016 |
Předmět: |
Epstein-Barr Virus Infections
Herpesvirus 4 Human Slovakia Cancer Research Pathology medicine.medical_specialty DNA polymerase Population In situ hybridization Malignancy medicine.disease_cause White People 03 medical and health sciences 0302 clinical medicine Keratinizing Squamous Cell Carcinoma hemic and lymphatic diseases medicine Humans 030223 otorhinolaryngology education Czech Republic Retrospective Studies education.field_of_study Nasopharyngeal Carcinoma biology business.industry Papillomavirus Infections virus diseases Nasopharyngeal Neoplasms medicine.disease Immunohistochemistry Virology Epstein–Barr virus Eastern european stomatognathic diseases Oncology Nasopharyngeal carcinoma 030220 oncology & carcinogenesis biology.protein RNA Viral business |
Zdroj: | Neoplasma. 63:107-114 |
ISSN: | 1338-4317 |
Popis: | Nasopharyngeal carcinoma (NPC) is a rare malignancy in the Czech Republic and Slovakia, with the standardized incidence rate of < 1:100000 person-years. Viral status of NPC in these non-endemic Eastern European regions is currently unknown. In a retrospective study, we evaluated the presence of EBV and HPV in 62 NPC cases. EBV status was determined by the use of in situ hybridization (ISH) for EBV encoded small RNA 1 (EBER1). HPV status was examined with p16 immunohistochemistry, DNA ISH and DNA polymerase chain reaction. Sixty-one studied cases showed non-keratinizing morphology and one was keratinizing squamous cell carcinoma. Only one NPC with non-keratinizing morphology was scored as p16-positive (nuclear and cytoplasmic staining ≥ 70% of tumor cells). This case was positive for high-risk HPV by ISH and the DNA PCR confirmed the presence of HPV18 type. At the same time, this case was found negative for EBV. Remaining sixty-one cases that were scored as p16-negative were all found HPV-negative by ISH and the DNA PCR. EBV was detected in 85.5% (53/62) of cases and 9 cases were EBV-negative, including the case of keratinizing NPC. In contrast with previous reports on the prevalence of EBV-positivity in Caucasian patients with NPC, the majority of patients coming from this non-endemic region show EBV-positivity; therefore, they may be candidates for novel EBV-targeting therapies. Conversely, HPV-positive NPC is very rare and HPV does not seem to play a significant role in the etiopathogenesis of NPC in these Eastern European populations. |
Databáze: | OpenAIRE |
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