Neurochemical Plasticity of nNOS-, VIP- and CART-Immunoreactive Neurons Following Prolonged Acetylsalicylic Acid Supplementation in the Porcine Jejunum
| Autor: | Jarosław Całka, Marta Czajkowska, Dominika Rząp |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Cart
pig medicine.medical_specialty Swine aspirin ENS Vasoactive intestinal peptide Myenteric Plexus Nerve Tissue Proteins Inflammation Nitric Oxide Synthase Type I Biology Neuroprotection Article Catalysis lcsh:Chemistry Inorganic Chemistry Neurochemical peripheral nervous system Internal medicine medicine Animals Physical and Theoretical Chemistry lcsh:QH301-705.5 Molecular Biology Spectroscopy Neurons Gastrointestinal tract Organic Chemistry General Medicine Computer Science Applications Jejunum medicine.anatomical_structure Endocrinology lcsh:Biology (General) lcsh:QD1-999 nervous system inflammation Peripheral nervous system Enteric nervous system neuroprotection medicine.symptom Vasoactive Intestinal Peptide |
| Zdroj: | International Journal of Molecular Sciences Volume 21 Issue 6 International Journal of Molecular Sciences, Vol 21, Iss 6, p 2157 (2020) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms21062157 |
| Popis: | Aspirin, also known as acetylsalicylic acid (ASA), is a commonly used anti-inflammatory drug that has analgesic and antipyretic properties. The side effects are well known, however, knowledge concerning its influence on gastric and intestinal innervation is limited. The enteric nervous system (ENS) innervates the whole gastrointestinal tract (GIT) and is comprised of more than one hundred million neurons. The capacity of neurons to adapt to microenvironmental influences, termed as an enteric neuronal plasticity, is an essential adaptive response to various pathological stimuli. Therefore, the goal of the present study was to determine the influence of prolonged ASA supplementation on the immunolocalization of neuronal nitric oxide synthase (nNOS), vasoactive intestinal peptide (VIP) and cocaine- and amphetamine- regulated transcript peptide (CART) in the porcine jejunum. The experiment was performed on 8 Pietrain × Duroc immature gilts. Using routine double-labelling immunofluorescence, we revealed that the ENS nerve cells underwent adaptive changes in response to the induced inflammation, which was manifested by upregulated or downregulated expression of the studied neurotransmitters. Our results suggest the participation of nNOS, VIP and CART in the development of inflammation and may form the basis for further neuro-gastroenterological research. |
| Databáze: | OpenAIRE |
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