The genome of a wild Medicago species provides insights into the tolerant mechanisms of legume forage to environmental stress
| Autor: | Lifei Ren, Caihong Li, Xiuxiu Zhang, Tianzuo Wang, Dan Gao, Mengyan Zhou, Rujin Chen, Feng-Ling Shi, Di Zhang, Nevin D. Young, Wen-Hao Zhang, Gang Zhou, Andrew Farmer, Zhaolan Wang, Yansu Li, Yuhui Chen |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Physiology Evolution QH301-705.5 Wild genetic resource Drought tolerance Plant Science 01 natural sciences Genome General Biochemistry Genetics and Molecular Biology Domestication 03 medical and health sciences Gene Expression Regulation Plant Stress Physiological Structural Biology Medicago ruthenica Medicago Medicago sativa Biology (General) Gene Ecology Evolution Behavior and Systematics 030304 developmental biology Single-nucleotide polymorphism Comparative genomics Genetics 0303 health sciences biology Abiotic stress fungi food and beverages Cell Biology biology.organism_classification Droughts Ruthenica General Agricultural and Biological Sciences Transcriptome Research Article 010606 plant biology & botany Developmental Biology Biotechnology |
| Zdroj: | BMC Biology, Vol 19, Iss 1, Pp 1-17 (2021) BMC Biology |
| ISSN: | 1741-7007 |
| Popis: | Background Medicago ruthenica, a wild and perennial legume forage widely distributed in semi-arid grasslands, is distinguished by its outstanding tolerance to environmental stress. It is a close relative of commonly cultivated forage of alfalfa (Medicago sativa). The high tolerance of M. ruthenica to environmental stress makes this species a valuable genetic resource for understanding and improving traits associated with tolerance to harsh environments. Results We sequenced and assembled genome of M. ruthenica using an integrated approach, including PacBio, Illumina, 10×Genomics, and Hi-C. The assembled genome was 904.13 Mb with scaffold N50 of 99.39 Mb, and 50,162 protein-coding genes were annotated. Comparative genomics and transcriptomic analyses were used to elucidate mechanisms underlying its tolerance to environmental stress. The expanded FHY3/FAR1 family was identified to be involved in tolerance of M. ruthenica to drought stress. Many genes involved in tolerance to abiotic stress were retained in M. ruthenica compared to other cultivated Medicago species. Hundreds of candidate genes associated with drought tolerance were identified by analyzing variations in single nucleotide polymorphism using accessions of M. ruthenica with varying tolerance to drought. Transcriptomic data demonstrated the involvements of genes related to transcriptional regulation, stress response, and metabolic regulation in tolerance of M. ruthenica. Conclusions We present a high-quality genome assembly and identification of drought-related genes in the wild species of M. ruthenica, providing a valuable resource for genomic studies on perennial legume forages. |
| Databáze: | OpenAIRE |
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