Molecular cloning, characterization and evolution of the gene encoding a new group of short-chain α-neurotoxins in an Australian elapid,Pseudonaja textilis
| Autor: | Kandiah Jeyaseelan, NanLing Gong, Arunmozhiarasi Armugam |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Chloramphenicol O-Acetyltransferase
Gene isoform Molecular Sequence Data Neurotoxins Biophysics CAAT box Reptilian Proteins Biology Polymerase Chain Reaction Biochemistry Evolution Molecular Pseudonaja textilis Exon Genes Reporter Structural Biology Genetics Animals Protein Isoforms Amino Acid Sequence RNA Messenger Cloning Molecular Promoter Regions Genetic Molecular Biology Gene Elapid Venoms Base Sequence Alternative splicing Intron Snakes Promoter Sequence Analysis DNA Cell Biology biology.organism_classification Alternative Splicing Sequence Alignment Snake Venoms |
| Zdroj: | FEBS Letters. 473:303-310 |
| ISSN: | 0014-5793 |
| Popis: | The structure and organization of five genes responsible for the synthesis of six isoforms of short-chain alpha-neurotoxins in Pseudonaja textilis venom are reported in this paper. This also forms the first report which describes the synthesis of two neurotoxin mRNA variants from one of these genes (Pt-sntx1) as a result of alternative splicing. Each gene consists of three exons which are separated by two introns and each has a functional promoter. The promoter activity was confirmed by both CAT assay and Real-Time PCR. A transcription initiation site, two putative TATA boxes, one CCAAT box and the transcription factor binding consensus sites for AP-1, GATA-2, c/EBPb were identified in the 5' non-coding region of each gene. Phylogenetic analysis showed that these five genes from P. textilis constituted a distinct group which has evolved by gene duplication followed by accelerated evolution from an ancestral gene. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text