Comparison of Polyacrylamide Gel Electrophoresis, an Enzyme-Linked-Immunosorbent Assay, and an Agglutination Test for the Direct Identification of Bovine Rotavirus from Feces and Coelectrophoresis of Viral RNA's
| Autor: | Anthony E. Castro, Bennie Osburn, Salah Hammami |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Rotavirus
0301 basic medicine 040301 veterinary sciences 030106 microbiology Cattle Diseases Reoviridae Enzyme-Linked Immunosorbent Assay Biology medicine.disease_cause Rotavirus Infections 0403 veterinary science Feces 03 medical and health sciences Predictive Value of Tests Direct agglutination test medicine Animals Polyacrylamide gel electrophoresis RNA Double-Stranded chemistry.chemical_classification General Veterinary 04 agricultural and veterinary sciences biology.organism_classification Molecular biology Latex fixation test Agglutination (biology) Enzyme chemistry RNA Viral Cattle Electrophoresis Polyacrylamide Gel Latex Fixation Tests |
| Zdroj: | Journal of Veterinary Diagnostic Investigation. 2:184-190 |
| ISSN: | 1943-4936 1040-6387 |
| DOI: | 10.1177/104063879000200306 |
| Popis: | The dsRNA concentrated polyacrylamide gel electrophoresis (CPAGE) detected rotavirus directly from 19% of 77 stool specimens from diarrheic calves. A commercial enzyme-linked immunosorbent assay (ELISA) detected 25%, latex agglutination test, 23%, and polyacrylamide gel electrophoresis (PAGE), 19%. Establishing CPAGE as the “standard,” the commercial ELISA and the latex agglutination test both had higher sensitivity (84%) than PAGE (79%). However, PAGE produced the highest specificity (100%), followed by agglutination (88%) and ELISA (84%). The commercial ELISA had a slightly higher sensitivity than agglutination, PAGE, and CPAGE, but the ELISA specificity was generally lower. The latex agglutination test had a lower sensitivity than ELISA, but specificity was higher. Agglutination had similar negative predictive values (94%), compared with agglutination and PAGE, but had the lowest positive predictive value (a measure of accuracy) (70%). Agreement with CPAGE was highest for PAGE (94.8%), followed by agglutination (87%) and ELISA (84.4%). The calculated percentages of total disagreement with all other tests indicated that ELISA differed from the other rotavirus detection assays in 10.4% of the cases, agglutination in 7.8%, PAGE in 2.6%, and CPAGE in 1.3%. The 2 PAGE assays allowed the detection of atypical rotaviruses from feces based on the characteristic “super-short” migration pattern of the 11 genomic segments of rotaviruses and of other members of the Reoviridae. |
| Databáze: | OpenAIRE |
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