Amyloid precursor protein, Aβ and amyloid-associated proteins involved in chloroquine retinopathy in rats – immunopathological studies
| Autor: | Yuji Aizawa, Naohiko Takahata, Nobuhiro Fujii, Nobuyuki Sasaki, Kayo Tsuzuki, Yuji Takamaru, Yorihide Hayashi, Ryo Fukatsu, Taku Yoshida |
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| Rok vydání: | 1997 |
| Předmět: |
Male
Retinal Ganglion Cells Apolipoprotein E Amyloid medicine.medical_specialty Pathology Cathepsin D Amyloid beta-Protein Precursor Retinal Diseases Internal medicine Amyloid precursor protein medicine Animals Rats Wistar Molecular Biology Ganglion cell layer Amyloid beta-Peptides biology General Neuroscience Chloroquine retinopathy Antibodies Monoclonal Chloroquine Inner plexiform layer medicine.disease Immunohistochemistry Rats medicine.anatomical_structure Endocrinology Retinal ganglion cell biology.protein sense organs Neurology (clinical) Developmental Biology |
| Zdroj: | Brain Research. 764:283-288 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/s0006-8993(97)00600-8 |
| Popis: | To understand the retinal changes in Alzheimer disease (AD) patients, pathological and immunocytochemical studies were performed on retinal cells in the chloroquine-treated rats at 0, 4, 8, 12, 16, 20, and 24 weeks after the initial injection, using anti-amyloid precursor protein (APP), -amyloid beta protein (A beta), -apolipoprotein E (apoE), -ubiquitin, and -cathepsin D antibodies. Pathological alterations consistent with chloroquine retinopathy were recognized in the ganglion cells of the ganglion cell layer (GCL) and the inner plexiform layer (IPL) 4 weeks after initial chloroquine injection. Rat retinal changes appear to have a direct relationship to the duration of chloroquine administration. Intense immunoreactivities for anti-APP, A beta, apoE (an associated protein), and ubiquitin co-localized in the swollen ganglion cells and Muller cells by 20-24 weeks together with the lysosomal enzyme cathepsin D. The present data indicate that the endosomal/lysosomal pathway plays an important role in the processing of APP in rat retina. This experimental model is considered to be a suitable neural model to understand retinal pathology and the processing of APP in terms of the pathogenesis of AD, whereas chloroquine-induced myopathy is a useful extra neuronal model. |
| Databáze: | OpenAIRE |
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