The Effect of Processing of Hempseed on Protein Recovery and Emulsification Properties
Autor: | Markus Nurmi, Nora Pap, Jarkko Mäkinen, Sari Mäkinen, Anne Pihlanto |
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Rok vydání: | 2021 |
Předmět: |
0106 biological sciences
genetic structures Article Subject Raw material 01 natural sciences Food processing and manufacture Defatting 0404 agricultural biotechnology 010608 biotechnology TX341-641 Food science Edestin 2. Zero hunger Gel electrophoresis Meal biology Nutrition. Foods and food supply Chemistry 04 agricultural and veterinary sciences TP368-456 040401 food science Supercritical fluid Germination Emulsion biology.protein Research Article Food Science |
Zdroj: | International Journal of Food Science, Vol 2021 (2021) International Journal of Food Science |
ISSN: | 2314-5765 2356-7015 |
DOI: | 10.1155/2021/8814724 |
Popis: | The effect of carbohydrate-hydrolysing enzyme blend with or without supercritical CO2 (SFE) defatting on pretreat hempseed meal, hempseeds, peeled hempseeds, hempseed protein powder, and germinated hempseeds was determined. The raw materials and recovered fractions from the treatments were subjected to gel electrophoresis, and their emulsion capacity, activity, and stability as well as colour (CIE L ∗ a ∗ b ∗ values) were determined. The highest protein contents, 65% ( w / w dm), were detected in soluble fractions prepared from germinated, defatted hempseeds followed by soluble fractions of peeled, defatted hempseed, 55% ( w / w dm). The gel electrophoresis showed quite similar protein profiles for all samples; however, the edestin content was lower in the germinated samples than in the others. Enzyme treatment and SFE did not have a significant effect on the emulsion properties. Germinated samples demonstrated a higher ability to stabilise emulsions (15-20%) than other pretreated samples. On the other hand, hempseed meal samples had lower emulsification activity and stability values compared to the other samples. The colour of the sample solutions varied from light to dark with a brown to yellowish colour, and PHS samples showed overall higher L ∗ values. In conclusion, germination and peeling in combination with defatting are promising methods to produce functional protein concentrates with efficient emulsion stability and activity as well as a mild colour for food applications. |
Databáze: | OpenAIRE |
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