Synchronization of excitable cardiac cultures of different origin
| Autor: | Carsten Kilgus, Tobias Bruegmann, V. A. Tsvelaya, Philipp Sasse, N. N. Agladze, O. V. Halaidych, Konstantin Agladze |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Primary culture Cell Biomedical Engineering chemistry.chemical_element 030204 cardiovascular system & hematology Biology Calcium Synchronization Cell Line 03 medical and health sciences 0302 clinical medicine medicine Fluorescence microscope Animals General Materials Science Myocytes Cardiac Neonatal rat Anatomy Electrophysiological Phenomena Rats 030104 developmental biology medicine.anatomical_structure chemistry Microscopy Fluorescence Cell culture Biophysics |
| Zdroj: | Biomaterials science. 5(9) |
| ISSN: | 2047-4849 |
| Popis: | In the present work, we investigated the synchronization of electrical activity in cultured cardiac cells of different origin put in direct contact. In the first set of experiments synchronization was studied in the primary culture cells of neonatal rats taken at different developmental ages, and in the second – in the neonatal rat cardiomyocytes and HL-1 cells. The electrical excitation of cells was recorded using the calcium transient marker Fluor-4. In the confluent cell layers created with the aid of a specially devised mask, the excitation waves and their propagation between areas occupied by cells of different origin were observed. On the level of individual cells, their contact and synchronization was monitored with the aid of scanning fluorescence microscopy. It was found that populations of cultured cells of different origin are able to synchronize, suggesting the formation of electrical coupling between them. The results obtained may be considered as a proof of concept that implanted alien grafted cells are able to create electrical coupling with the host cardiac tissue. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|