Intact Purine Biosynthesis Pathways Are Required for Wild-Type Virulence of Brucella abortus 2308 in the BALB/c Mouse Model
| Autor: | Rosemarie B. Alcantara, Michelle Wright Valderas, Richard D. A. Read, Timothy D. Brown, R. Martin Roop |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Purine
BALB/c Mouse Immunology Mutant Brucella abortus Virulence Microbiology Brucellosis Mice chemistry.chemical_compound Bacterial Proteins Biosynthesis Animals Humans Purine metabolism Cells Cultured Mice Inbred BALB C biology Wild type Bacterial Infections biology.organism_classification Culture Media Disease Models Animal Mutagenesis Insertional Infectious Diseases chemistry Purines DNA Transposable Elements Macrophages Peritoneal Parasitology Brucella melitensis |
| Zdroj: | Infection and Immunity. 72:4911-4917 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.72.8.4911-4917.2004 |
| Popis: | Brucella abortus 2308 derivatives with mini-Tn 5 insertions in purE , purL , and purD display significant attenuation in the BALB/c mouse model, while isogenic mutants with mini-Tn 5 insertions in pheA , trpB , and dagA display little or no attenuation in cultured murine macrophages or mice. These experimental findings confirm the importance of the purine biosynthesis pathways for the survival and replication of the brucellae in host macrophages. In contrast to previous reports, however, these results indicate that exogenous tryptophan and phenylalanine are available for use by the brucellae in the phagosomal compartment. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|