Mutational analysis indicates that abnormalities in rhizobial infection and subsequent plant cell and bacteroid differentiation in pea (Pisum sativum) nodules coincide with abnormal cytokinin responses and localization
| Autor: | Anna N. Kirienko, Anna V. Tsyganova, Viktor E. Tsyganov, P. G. Kusakin, Kirill N. Demchenko, I. V. Leppyanen, Aleksandra V. Dolgikh, Elena L. Ilina, Igor A. Tikhonovich, Anna B. Kitaeva, Elena A. Dolgikh |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Cytokinins Mutant Plant Science Infections 01 natural sciences Plant Roots Pisum Nod factor 03 medical and health sciences chemistry.chemical_compound Auxin Gene Expression Regulation Plant Plant Cells medicine Humans heterocyclic compounds Symbiosis chemistry.chemical_classification biology fungi Wild type Peas food and beverages Nodule (medicine) Cell Differentiation Original Articles Meristem biology.organism_classification Cell biology 030104 developmental biology chemistry Cytokinin Mutation medicine.symptom 010606 plant biology & botany Rhizobium |
| Zdroj: | Ann Bot |
| Popis: | Background and AimsRecent findings indicate that Nod factor signalling is tightly interconnected with phytohormonal regulation that affects the development of nodules. Since the mechanisms of this interaction are still far from understood, here the distribution of cytokinin and auxin in pea (Pisum sativum) nodules was investigated. In addition, the effect of certain mutations blocking rhizobial infection and subsequent plant cell and bacteroid differentiation on cytokinin distribution in nodules was analysed.MethodsPatterns of cytokinin and auxin in pea nodules were profiled using both responsive genetic constructs and antibodies.Key ResultsIn wild-type nodules, cytokinins were found in the meristem, infection zone and apical part of the nitrogen fixation zone, whereas auxin localization was restricted to the meristem and peripheral tissues. We found significantly altered cytokinin distribution in sym33 and sym40 pea mutants defective in IPD3/CYCLOPS and EFD transcription factors, respectively. In the sym33 mutants impaired in bacterial accommodation and subsequent nodule differentiation, cytokinin localization was mostly limited to the meristem. In addition, we found significantly decreased expression of LOG1 and A-type RR11 as well as KNOX3 and NIN genes in the sym33 mutants, which correlated with low cellular cytokinin levels. In the sym40 mutant, cytokinins were detected in the nodule infection zone but, in contrast to the wild type, they were absent in infection droplets.ConclusionsIn conclusion, our findings suggest that enhanced cytokinin accumulation during the late stages of symbiosis development may be associated with bacterial penetration into the plant cells and subsequent plant cell and bacteroid differentiation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|