Impact of microparticles derived from erythrocytes on fibrinolysis
| Autor: | Grigory Levin, Athina Lavrentieva, Ekaterina Sukhareva |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Male
Erythrocytes medicine.medical_treatment 030204 cardiovascular system & hematology Fibrin 03 medical and health sciences 0302 clinical medicine Cell-Derived Microparticles Fibrinolysis medicine Humans Whole blood biology business.industry Chromogenic Substrate (chemistry) Hematology Red blood cell medicine.anatomical_structure Coagulation Blood Preservation Hemostasis Immunology biology.protein Biophysics Female Cardiology and Cardiovascular Medicine business 030215 immunology |
| Zdroj: | Journal of thrombosis and thrombolysis. 41(3) |
| ISSN: | 1573-742X |
| Popis: | It has long been known that negatively charged membranes of erythrocyte-derived microparticles display procoagulant activity. However, relatively little is known about the possible fibrinolytic activity of such microparticles. This issue becomes particularly important during red blood cell storage, which significantly increases the number of microparticles. Whole blood was collected from 30 healthy donors. Microparticles were isolated on days 7, 14, 21, and 28 of erythrocyte storage. The effect of microparticles on the fibrinolytic activity of the donor plasma was determined by coagulation and optical (chromogenic substrate) methods. We demonstrated that erythrocyte microparticles had a prominent fibrinolytic activity which cleaves not only fibrin but also chromogenic substrates. Microparticles present fibrinolytic activity mainly due to the presence of plasminogen on them. Microparticles derived from erythrocytes significantly enhance cleavage of the chromogenic substrate by the streptokinase-plasminogen complex, but to a lesser extent accelerate euglobulin clot lysis time. Erythrocyte-derived microparticles display prominent fibrinolytic activity, which significantly decreases during storage of red blood cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink