InterSEPTIN' Kinesins in Dendrites
| Autor: | Santiago Balseiro Gómez, Shaul Yogev |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Neurons Developmental cell Motility Kinesins Cell Biology macromolecular substances Dendrites Biology Microtubules General Biochemistry Genetics and Molecular Biology Axons Article Cell biology 03 medical and health sciences 030104 developmental biology book.journal Kinesin Neuronal polarity Molecular Biology book Function (biology) Developmental Biology |
| Zdroj: | Developmental cell. 46(2) |
| ISSN: | 1878-1551 |
| Popis: | Neuronal function requires axon-dendrite membrane polarity, which depends on sorting of membrane traffic during entry into axons. Due to a microtubule network of mixed polarity, dendrites receive vesicles from the cell body without apparent capacity for directional sorting. We found that, during entry into dendrites, axonally destined cargos move with a retrograde bias toward the cell body, while dendritically destined cargos are biased in the anterograde direction. A microtubule-associated septin (SEPT9), which localizes specifically in dendrites, impedes axonal cargo of kinesin-1/KIF5 and boosts kinesin-3/KIF1 motor cargo further into dendrites. In neurons and in vitro single-molecule motility assays, SEPT9 suppresses kinesin-1/KIF5 and enhances kinesin-3/KIF1 in a manner that depends on a lysine-rich loop of the kinesin motor domain. This differential regulation impacts partitioning of neuronal membrane proteins into axons-dendrites. Thus, polarized membrane traffic requires sorting during entry into dendrites by a septin-mediated mechanism that bestows directional bias on microtubules of mixed orientation. |
| Databáze: | OpenAIRE |
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