Colonization and growth of dehalorespiring biofilms on carbonaceous sorptive amendments
| Autor: | Birthe V. Kjellerup, Kevin R. Sowers, Staci L. Capozzi, Rayford B. Payne, Coline Bodenreider, Ana L. Prieto |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Geologic Sediments Halogenation 030106 microbiology Biological Availability Aquatic Science Applied Microbiology and Biotechnology Polymerase Chain Reaction 03 medical and health sciences Food chain Adsorption medicine Sediment contamination Colonization Biomass Water Science and Technology Microscopy Confocal Chemistry organic chemicals Biofilm food and beverages Chloroflexi Contamination Polychlorinated Biphenyls Carbon Bioavailability 030104 developmental biology Environmental chemistry Biofilms Charcoal Microscopy Electron Scanning Chlorine Activated carbon medicine.drug |
| DOI: | 10.6084/m9.figshare.7751174 |
| Popis: | Removal of polychlorinated biphenyls (PCBs) from contaminated sediments is a priority due to accumulation in the food chain. Recent success with reduction of PCB bioavailability due to adsorption onto activated carbon led to the recognition of in situ treatment as a remediation approach. In this study, reduced bioavailability and subsequent break-down of PCBs in dehalorespiring biofilms was investigated using Dehalobium chlorocoercia DF1. DF1 formed a patchy biofilm ranging in thickness from 3.9 to 6.7 µm (average 4.6 ± 0.87 µm), while the biofilm coverage varied from 5.5% (sand) to 20.2% (activated carbon), indicating a preference for sorptive materials. Quantification of DF1 biofilm bacteria showed 1.2–15.3 × 109 bacteria per gram of material. After 22 days, coal activated carbon, bone biochar, polyoxymethylene, and sand microcosms had dechlorinated 73%, 93%, 100%, and 83%, respectively. These results show that a biofilm-based inoculum for bioaugmentation of PCBs in sediment can be an efficient approach. |
| Databáze: | OpenAIRE |
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