Aqueous and methanol extracts of Paullinia pinnata L. (Sapindaceae) improve inflammation, pain and histological features in CFA-induced mono-arthritis: Evidence from in vivo and in vitro studies
| Autor: | Zacharie Sando, Judith Manialeu Pouadjeu, Basile Nganmegne Piegang, Télesphore Benoît Nguelefack, Kevin Tidgewell, Benedict J. Kolber, Douglas Alphonse Mouga Ngangoum, Pius Pum Tseuguem |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
medicine.drug_class Analgesic Freund's Adjuvant Anti-Inflammatory Agents Drug Evaluation Preclinical Arthritis Pharmacology Nitric Oxide Anti-inflammatory Article Arthritis Rheumatoid 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine In vivo Drug Discovery medicine Monoarthritis Paullinia Animals Humans Rats Wistar Medicine African Traditional 030304 developmental biology Paullinia pinnata 0303 health sciences Analgesics biology Plant Extracts Methanol Water medicine.disease Malondialdehyde biology.organism_classification Arthralgia Arthritis Experimental In vitro Rats Plant Leaves Treatment Outcome chemistry 030220 oncology & carcinogenesis Female Ankle Joint |
| Zdroj: | J Ethnopharmacol |
| ISSN: | 1872-7573 |
| Popis: | ETHNOPHARMACOLOGICAL RELEVANCE: Paullinia pinnata L. (Sapindaceae) is an African woody vine, traditionally used for the treatment of itch and pain-related conditions such as rheumatoid arthritis. AIM: This work evaluates, in vitro and in vivo, the anti-inflammatory and analgesic effects of aqueous (AEPP) and methanol (MEPP) extracts from Paullinia pinnata leaves. METHODS: AEPP and MEPP (100, 200 and 300 mg/kg/day) were administered orally in monoarthritic rats induced by a unilateral injection of 50 μl of Complete Freund’s Adjuvant (CFA) in the ankle joint. During the 14 days of treatment, pain and inflammation were evaluated alternatively in both ankle and paw of the CFA-injected leg. Malondialdehyde (MDA) and glutathione (GSH) levels were assessed in serum and spinal cord. Histology of smooth tissue of the paw was also analyzed. For in vitro studies, AEPP and MEPP (10, 30 and 100 μg/ml) were evaluated against nitric oxide (NO) production by macrophages that were either non-stimulated or stimulated with LPS, 8-Br-AMPc and the mixture of both substances after 8h exposure. These extracts were also evaluated on TNF-α and IL-1β production in cells stimulated with LPS for 8 hours. Finally, the ability of the extracts to bind to neuroactive receptors was evaluated in vitro using competitive binding assays with >45 molecular targets. RESULTS: AEPP and MEPP significantly reduced by 20 to 98% (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect