Characterization of a new endoglucanase from Erwinia chrysanthemi
| Autor: | Jean-Pierre Chambost, Brigitte Cami, Marie-Helene Boyer, Jeanne Cattanéo, Mireille Magnan |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
biology
Transcription Genetic Chemistry Structural gene Nucleic Acid Hybridization Periplasmic space DNA Restriction Enzymes Erwinia biology.organism_classification medicine.disease_cause Biochemistry Molecular biology Gene product Plasmid Cellulase Genes Genes Bacterial medicine Cosmid Escherichia coli bacteria Gene Plasmids |
| Zdroj: | European journal of biochemistry. 162(2) |
| ISSN: | 0014-2956 |
| Popis: | The structural gene coding for a new endo-beta-1,4-glucanase of Erwinia chrysanthemi strain 3665, previously identified in a cosmid library, was subcloned into pUC18. The gene is expressed from a 1.9 X 10(3)-base-pair insert and its direction of transcription was determined. The properties of the gene product purified from cell-free extracts of Escherichia coli have been studied. The purified protein has an endoglucanase activity but is significantly different from the major endoglucanase Z secreted by E. chrysanthemi strain 3665. The new enzyme was designated as endoglucanase Y and the related gene celY. In E. coli, most of the endoglucanase activity was found in the periplasmic space. |
| Databáze: | OpenAIRE |
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