Multimodal molecular imaging system for pathway-specific reporter gene expression
| Autor: | Silvia Valensin, Enrica Diodato, Elena Pilli, Carla Scali, Luisa Massai, Roberta Magrini, Riccardo Roggeri, Antonella De Rosa, Fabio A. Recchia, Nicola P. Caradonna, Sara Chellini, Giuseppe Pollio, Patrizia Tunici, Valentina Porcari, Gianluca Sardone, Pasquale Fiengo, Letizia Magnoni, Silvia Coniglio, Vincenzo Lionetti, Daniela Diamanti, Martina Monti, Marco Rossi |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Nude Pharmaceutical Science Gene Expression Mice Nude Biology Cell Line Mice 03 medical and health sciences In vivo Genes Reporter Luciferases Firefly Multimodal imaging Cell Line Tumor medicine Animals Humans Luciferase Luciferases Reporter Firefly Wnt Signaling Pathway Reporter gene Tumor medicine.diagnostic_test BLI Molecular Imaging MRI Apoferritins Brain Female Lithium Chloride Magnetic Resonance Imaging Optical Imaging 3003 Magnetic resonance imaging Transplantation 030104 developmental biology Genes Molecular imaging Preclinical imaging Ex vivo |
| Zdroj: | European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences. 86 |
| ISSN: | 1879-0720 |
| Popis: | Preclinical imaging modalities represent an essential tool to develop a modern and translational biomedical research. To date, Optical Imaging (OI) and Magnetic Resonance Imaging (MRI) are used principally in separate studies for molecular imaging studies. We decided to combine OI and MRI together through the development of a lentiviral vector to monitor the Wnt pathway response to Lithium Chloride (LiCl) treatment. The construct was stably infected in glioblastoma cells and, after intracranial transplantation in mice, serial MRI and OI imaging sessions were performed to detect human ferritin heavy chain protein (hFTH) and firefly luciferase enzyme (FLuc) respectively. The system allowed also ex vivo analysis using a constitutive fluorescence protein expression. In mice, LiCl administration has shown significantly increment of luminescence signal and a lower signal of T2 values (P |
| Databáze: | OpenAIRE |
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