Palmitate protects hepatocytes from oxidative stress and triacylglyceride accumulation by stimulation of nitric oxide synthesis in the presence of high glucose and insulin concentration
| Autor: | Gerburg Keilhoff, Müller Christian, Lorenz Schild, Andreas Gardemann, Siegfried Kropf, Daniela Peter, Ingrid Wiswedel |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Male
medicine.medical_specialty Cardiolipins Cell Survival medicine.medical_treatment Linoleic acid Palmitates Nitric Oxide Synthase Type II Stimulation Nitric Oxide medicine.disease_cause Biochemistry Nitric oxide chemistry.chemical_compound Physiology (medical) Internal medicine medicine Cardiolipin Animals Insulin Rats Wistar Nitrite Cells Cultured Triglycerides F2-Isoprostanes Arachidonic Acid Nitric oxide synthesis biology Fatty liver General Medicine medicine.disease Mitochondria Rats Nitric oxide synthase Oxidative Stress Glucose Endocrinology Linoleic Acids chemistry High glucose Hepatocytes biology.protein Oxidative stress |
| Zdroj: | Free Radical Research. 44:1425-1434 |
| ISSN: | 1029-2470 1071-5762 |
| DOI: | 10.3109/10715762.2010.512919 |
| Popis: | Excessive flux of free fatty acids (FFA) into the liver contributes to liver impairment in non-alcoholic fatty liver disease (NAFLD). It remains unclear how FFA contribute to impairment of hepatocytes. This study treated hepatocytes with linoleic acid and palmitate to investigate the early event triggering FFA-mediated impairment. It determined cell viability, content of nitrite/nitrate and triacylglycerides (TG), inducible nitric oxide synthase (iNOS) protein, oxidation of cardiolipin (CL) as well as formation of F(2)-isoprostanes in the presence of insulin and glucose. Linoleic acid caused significant decrease in cell viability. It is shown that palmitate caused induction of iNOS resulting in increased nitrite/nitrate concentration and slight increase in TG content. Linoleic acid led to a decrease in nitrite/nitrate concentration parallelled by massive TG accumulation in combination with increased oxidation of CL and increased F(2)-isoprostane levels. It is concluded that nitric oxide (NO) concentration regulates FFA-dependent TG accumulation and oxidative stress in rat hepatocytes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |