In Vitro Assessment of Poly-N-Vinylpyrrolidone/Acrylic Acid Nanoparticles Biocompatibility in a Microvascular Endothelium Model

Autor:	Aikaterini Berdiaki, Andrey N. Kuskov, Pavel P. Kulikov, Lydia-Nefeli Thrapsanioti, Eirini-Maria Giatagana, Polychronis Stivaktakis, Mikhail I. Shtilman, Aristidis Tsatsakis, Dragana Nikitovic
Rok vydání:	2022
Předmět:	Lipopolysaccharides Polymers Organic Chemistry Endothelial Cells poly-N-vinylpyrrolidone acrylic acid amphiphilic polymer nanoparticle endothelial cells assessment viability immunological activation fluorescent probes General Medicine Intercellular Adhesion Molecule-1 Catalysis Computer Science Applications Inorganic Chemistry Humans Nanoparticles Endothelium Physical and Theoretical Chemistry E-Selectin Molecular Biology Spectroscopy
Zdroj:	International Journal of Molecular Sciences; Volume 23; Issue 20; Pages: 12446
ISSN:	1422-0067
DOI:	10.3390/ijms232012446
Popis:	An amphiphilic copolymer of N-vinyl-2-pyrrolidone and acrylic acid—namely, p(VP-AA)-OD6000 (p(VP-AA))—was synthesized to prepare p(VP-AA) nanoparticles (NPs). Furthermore, the copolymer was linked with CFSE, and the so-prepared nanoparticles were loaded with the DiI dye to form D nanoparticles (DNPs). In this study, as demonstrated by immunofluorescence microscopy, immunofluorescence, and confocal microscopy, DNPs were readily taken up by human microvascular endothelial cells (HMEC-1) cells in a concentration-dependent manner. Upon uptake, both the CFSE dye (green stain) and the DiI dye (red stain) were localized to the cytoplasm of treated cells. Treatment with p(VP-AA) did not affect the viability of normal and challenged with LPS, HMEC-1 cells at 0.010 mg/mL and induced a dose-dependent decrease of these cells’ viability at the higher concentrations of 0.033 and 0.066 mg/mL (p ≤ 0.01; p ≤ 0.001, respectively). Furthermore, we focused on the potential immunological activation of HMEC-1 endothelial cells upon p(VP-AA) NPs treatment by assessing the expression of adhesion molecules (E-Selectin, ICAM-1, and V-CAM). NPs treatments at concentrations utilized (p = NS) did not affect individual adhesion molecules’ expression. p(VP-AA) NPs do not activate the endothelium and do not affect its viability at pharmacologically relevant concentrations.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eef1a7b326f7a868d5a6394b2eeda1dc https://doi.org/10.3390/ijms232012446 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.