Prevention of antibiotic-associated metabolic syndrome in mice by intestinal alkaline phosphatase
Autor: | S. Hakimian, Rizwan Ahmed, Mussa M. Rafat Mohamed, Kanakaraju Kaliannan, Palak Patel, Omeed Moaven, Caleb D. Phillips, Konstantinos P. Economopoulos, Sayeda Nasrin Alam, Naomi L. Ward, Madhu S. Malo, J. F. Haller, Abeba Teshager, Stephen B. Cox, Richard A. Hodin, Atul K. Bhan, Allan M. Goldstein |
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Rok vydání: | 2015 |
Předmět: |
0301 basic medicine
Male medicine.medical_specialty viruses Endocrinology Diabetes and Metabolism Blood lipids Weaning Gut flora Azithromycin Diet High-Fat Weight Gain Article 03 medical and health sciences Feces 0302 clinical medicine Endocrinology Intestinal mucosa Internal medicine Internal Medicine Medicine Animals Bacteroides Obesity Intestinal Mucosa Metabolic Syndrome biology business.industry medicine.disease biology.organism_classification Alkaline Phosphatase Anti-Bacterial Agents Gastrointestinal Microbiome Mice Inbred C57BL Molecular Typing 030104 developmental biology Immunology Dietary Supplements Alkaline phosphatase Dysbiosis Cattle Metabolic syndrome medicine.symptom business Weight gain 030217 neurology & neurosurgery Acholeplasma |
Zdroj: | Diabetes, obesitymetabolism. 18(5) |
ISSN: | 1463-1326 |
Popis: | Aims To examine whether co-administration of intestinal alkaline phosphatase (IAP) with antibiotics early in life may have a preventive role against metabolic syndrome (MetS) in mice. Methods A total of 50 mice were allocated to four treatment groups after weaning. Mice were treated with azithromycin (AZT) ± IAP, or with no AZT ± IAP, for three intermittent 7-day cycles. After the last treatment course, the mice were administered a regular chow diet for 5 weeks and subsequently a high-fat diet for 5 weeks. Body weight, food intake, water intake, serum lipids, glucose levels and liver lipids were compared. 16S rRNA gene pyrosequencing was used to determine the differences in microbiome composition. Results Exposure to AZT early in life rendered mice susceptible to MetS in adulthood. Co-administration of IAP with AZT completely prevented this susceptibility by decreasing total body weight, serum lipids, glucose levels and liver lipids to the levels of control mice. These effects of IAP probably occur as a result of changes in the composition of specific bacterial taxa at the genus and species levels (e.g. members of Anaeroplasma and Parabacteroides). Conclusions Co-administration of IAP with AZT early in life prevents mice from susceptibility to the later development of MetS. This effect is associated with alterations in the composition of the gut microbiota. IAP may represent a novel treatment against MetS in humans. |
Databáze: | OpenAIRE |
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