Identification of potential candidate proteins for reprogramming spinal cord-derived astrocytes into neurons: a proteomic analysis
Autor: | Yuxiang Lin, Shu-Xia Xu, Wen-Hao Chen, Ling Lin, Bao-Quan Zhang, Wei Wang |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Casein Kinase II Subunit Alpha
mechanism neurons label-free proteomic analysis Biology lcsh:RC346-429 Developmental Neuroscience In vivo medicine Spinal cord injury lcsh:Neurology. Diseases of the nervous system primary culture astrocytes reprogramming Neurogenic Differentiation Factor 1 medicine.disease Spinal cord spinal cord injury In vitro glial cells Cell biology medicine.anatomical_structure NEUROD1 Reprogramming Research Article |
Zdroj: | Neural Regeneration Research, Vol 16, Iss 11, Pp 2257-2263 (2021) Neural Regeneration Research |
ISSN: | 1673-5374 |
Popis: | Our previous study has confirmed that astrocytes overexpressing neurogenic differentiation factor 1 (NEUROD1) in the spinal cord can be reprogrammed into neurons under in vivo conditions. However, whether they can also be reprogrammed into neurons under in vitro conditions remains unclear, and the mechanisms of programmed conversion from astrocytes to neurons have not yet been clarified. In the present study, we prepared reactive astrocytes from newborn rat spinal cord astrocytes using the scratch method and infected them with lentivirus carrying NEUROD1. The results showed that NEUROD1 overexpression reprogrammed the cultured reactive astrocytes into neurons in vitro with an efficiency of 13.4%. Using proteomic and bioinformatic analyses, 1952 proteins were identified, of which 92 were differentially expressed. Among these proteins, 11 were identified as candidate proteins in the process of reprogramming based on their biological functions and fold-changes in the bioinformatic analysis. Furthermore, western blot assay revealed that casein kinase II subunit alpha (CSNK2A2) and pinin (PNN) expression in NEUROD1-overexpressing reactive astrocytes was significantly increased, suggesting that NEUROD1 can directly reprogram spinal cord-derived reactive astrocytes into neurons in vitro, and that the NEUROD1-CSNK2A2-PNN pathway is involved in this process. This study was approved by the Animal Ethics Committee of Fujian Medical University, China (approval No. 2016-05) on April 18, 2016. |
Databáze: | OpenAIRE |
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