Re-sequencing of DNA from a diverse panel of cattle reveals a high level of polymorphism in both intron and exon
| Autor: | B A Konfortov, Victoria E. Licence, J. Ross Miller |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Leptin
Sequence analysis Single-nucleotide polymorphism Biology Chromosomes DNA sequencing Amyloid beta-Protein Precursor chemistry.chemical_compound Exon Gene Frequency Genetics Animals Humans Gene Polymorphism Genetic Nucleic acid sequence Intron Genetic Variation Exons Sequence Analysis DNA Molecular biology Introns Amino Acid Substitution chemistry Mutation Cattle DNA |
| Zdroj: | Mammalian Genome. 10:1142-1145 |
| ISSN: | 1432-1777 0938-8990 |
| DOI: | 10.1007/s003359901180 |
| Popis: | In order to assess the extent of DNA sequence variation in cattle, introns and exons from both the leptin and Amyloid Precursor Protein (APP) genes have been sequenced in a panel of DNAs derived from 22 diverse animals. Direct DNA sequencing of PCR products was used; thus, 44 chromosomes were studied. Polymorphisms were identified by manual scanning of sequence chromatograms and computerized sequence analysis. Twenty Single Nucleotide Polymorphisms (SNPs) were detected in 1788 bp sequenced from the leptin gene, giving a frequency of 1 SNP per 89 bp. Twenty-four SNPs were detected in a 458-bp fragment of the APP gene; 23 of the polymorphisms were contained in a 302-bp intron 16 fragment. This equates to an SNP frequency of 1 per 13 bp for the intron. We can thus conclude that this portion of the bovine APP gene constitutes a hypermutable region. Nucleotide sequence diversity values of 0.019 and 0.0026 were obtained for APP and leptin respectively. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink