Use of INT to determine the respiratory activity of immobilised and free Penicillium chrysogenum
| Autor: | Christopher Bucke, Tajalli Keshavarz, Paul J. Mussenden, Gunter Saunders |
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| Rok vydání: | 1994 |
| Předmět: |
Cellular respiration
General Chemical Engineering Tetrazolium Salts Biology Penicillium chrysogenum law.invention Inorganic Chemistry chemistry.chemical_compound law Waste Management and Disposal Clark electrode Staining and Labeling Renewable Energy Sustainability and the Environment Tetrazolium chloride Organic Chemistry INT Metabolism biology.organism_classification Pollution Molecular biology Staining Oxygen Fuel Technology chemistry Biochemistry Cell culture Fermentation Biotechnology |
| Zdroj: | Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986). 60(1) |
| ISSN: | 0268-2575 |
| Popis: | The specific oxygen uptake rates (OUR) of kappa-carrageenan-immobilised and free cell cultures of Penicillium chrysogenum were determined using an oxygen electrode in a closed chamber. This was compared with the respiratory activity determined by the extent of staining with iodo-nitrophenyl tetrazolium chloride (INT). The degree of INT staining correlated with the OUR; an increase in INT deposition corresponding to an increase in the measured OUR. The INT staining technique could therefore be used to determine cell respiratory activity. In this way a profile of fungal cell activity throughout immobilised cell aggregates and free cell pellets was determined. In both types of cell culture, after the initial growth period only the peripheral biomass was observed to be active. |
| Databáze: | OpenAIRE |
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